Gupta V P, Keiderling T A
Department of Chemistry, University of Illinois, Chicago 60680.
Biopolymers. 1992 Mar;32(3):239-48. doi: 10.1002/bip.360320305.
The amide II vibrational CD (VCD) spectra of poly (L-glutamic acid) and poly (L-lysine) in various conformational forms and those of several proteins in H2O have been measured. Characteristic VCD patterns have been observed in the amide II region due to helix, beta-sheet, and coil conformations in polypeptides. Based on their x-ray crystal structures, the proteins studied have been assigned to six categories. Proteins in the same category give rise to similar amide II VCD. While the protein conformational type is indicated using the amide II VCD, discrimination between types is less characteristic than with the previously studied amide I' VCD in D2O.
已测量了处于各种构象形式的聚（L-谷氨酸）和聚（L-赖氨酸）以及几种蛋白质在H₂O中的酰胺II振动圆二色性（VCD）光谱。由于多肽中的螺旋、β-折叠和无规卷曲构象，在酰胺II区域观察到了特征性的VCD模式。基于它们的X射线晶体结构，所研究的蛋白质被分为六类。同一类别的蛋白质会产生相似的酰胺II VCD。虽然使用酰胺II VCD来指示蛋白质的构象类型，但与之前在D₂O中研究的酰胺I' VCD相比，不同类型之间的区分特征较弱。
