钠离子对L型钙通道的调节作用。
Modulation of L-type calcium channels by sodium ions.
作者信息
Balke C W, Wier W G
机构信息
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
出版信息
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4417-21. doi: 10.1073/pnas.89.10.4417.
Abstract
It is universally believed that the removal of external sodium ions is without effect on calcium current. We now report that in enzymatically isolated guinea pig ventricular cells, the replacement of external sodium ions with certain other cations causes a 3- to 6-fold increase in peak L-type calcium current. The increase in current is reversibly blocked by L-type calcium-channel antagonists, not mediated by changes in internal calcium, and is inhibited by intracellular 5'-adenylyl imidodiphosphate, a nonhydrolyzable ATP analogue. The effects of sodium removal (and isoproterenol) were almost completely blocked by intracellular application of a specific (peptide) inhibitor of cAMP-dependent protein kinase. These experiments demonstrate a previously unknown effect of sodium ions to modulate calcium-channel phosphorylation via cAMP-dependent protein kinase.
摘要
人们普遍认为，去除细胞外钠离子对钙电流没有影响。我们现在报告，在酶解分离的豚鼠心室细胞中，用某些其他阳离子替代细胞外钠离子会使L型钙电流峰值增加3至6倍。电流的增加可被L型钙通道拮抗剂可逆性阻断，不是由细胞内钙的变化介导的，并且被细胞内5'-腺苷酰亚胺二磷酸（一种不可水解的ATP类似物）抑制。去除钠离子（以及异丙肾上腺素）的作用几乎完全被细胞内应用的一种特异性（肽）cAMP依赖性蛋白激酶抑制剂所阻断。这些实验证明了钠离子通过cAMP依赖性蛋白激酶调节钙通道磷酸化这一先前未知的作用。
相似文献
1
Modulation of L-type calcium channels by sodium ions.钠离子对L型钙通道的调节作用。
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4417-21. doi: 10.1073/pnas.89.10.4417.
2
Background inward current in ventricular and atrial cells of the guinea-pig.
Proc Biol Sci. 1993 Apr 22;252(1333):65-74. doi: 10.1098/rspb.1993.0047.
3
Intracellular Na+ modulates the cAMP-dependent regulation of ion channels in the heart.细胞内钠离子调节心脏中离子通道的环磷酸腺苷（cAMP）依赖性调控。
Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):6946-50. doi: 10.1073/pnas.88.16.6946.
4
An isoprenaline activated sodium-dependent inward current in ventricular myocytes.
Nature. 1987;328(6131):634-7. doi: 10.1038/328634a0.
5
A novel, voltage-dependent nonselective cation current activated by insulin in guinea pig isolated ventricular myocytes.一种由胰岛素激活的新型、电压依赖性非选择性阳离子电流，存在于豚鼠离体心室肌细胞中。
Circ Res. 2003 Apr 18;92(7):765-8. doi: 10.1161/01.RES.0000065920.64121.FC. Epub 2003 Mar 13.
6
Modulation of L-type Ca2+ current by fast and slow Ca2+ buffering in guinea pig ventricular cardiomyocytes.豚鼠心室肌细胞中快速和慢速Ca2+缓冲对L型Ca2+电流的调节作用
Biophys J. 1997 Jan;72(1):175-87. doi: 10.1016/S0006-3495(97)78656-9.
7
Regulation of cardiac sodium-calcium exchanger by beta-adrenergic agonists.β-肾上腺素能激动剂对心脏钠钙交换体的调节作用。
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5527-32. doi: 10.1073/pnas.93.11.5527.
8
Genistein directly inhibits L-type calcium currents but potentiates cAMP-dependent chloride currents in cardiomyocytes.染料木黄酮直接抑制心肌细胞中的L型钙电流，但增强cAMP依赖性氯电流。
Biochem Biophys Res Commun. 1996 Jun 25;223(3):598-603. doi: 10.1006/bbrc.1996.0941.
9
Mechanism of ion permeation through calcium channels.离子通过钙通道的渗透机制。
Nature. 1984;309(5967):453-6. doi: 10.1038/309453a0.
10
A new calcium current underlying the plateau of the cardiac action potential.一种构成心脏动作电位平台期基础的新钙电流。
Proc R Soc Lond B Biol Sci. 1984 Nov 22;223(1230):35-48. doi: 10.1098/rspb.1984.0081.
引用本文的文献
1
The Ca transient as a feedback sensor controlling cardiomyocyte ionic conductances in mouse populations.钙离子瞬变作为一种反馈传感器，控制小鼠群体心肌细胞离子电导。
Elife. 2018 Sep 25;7:e36717. doi: 10.7554/eLife.36717.
2
An antisense oligonucleotide against H1 inhibits the classical sodium current but not ICa(TTX) in rat ventricular cells.针对H1的反义寡核苷酸可抑制大鼠心室细胞中的经典钠电流，但不影响ICa（TTX）。
J Physiol. 2003 Mar 1;547(Pt 2):435-40. doi: 10.1113/jphysiol.2002.035246. Epub 2003 Jan 24.
3
I(Ca(TTX)) channels are distinct from those generating the classical cardiac Na(+) current.I(Ca(TTX))通道与产生经典心脏钠电流的通道不同。
Biophys J. 2001 Nov;81(5):2647-59. doi: 10.1016/s0006-3495(01)75908-5.
4
Tetrodotoxin-blockable calcium currents in rat ventricular myocytes; a third type of cardiac cell sodium current.大鼠心室肌细胞中可被河豚毒素阻断的钙电流；第三种类型的心肌细胞钠电流。
J Physiol. 1997 Dec 1;505 ( Pt 2)(Pt 2):353-69. doi: 10.1111/j.1469-7793.1997.353bb.x.
5
Disruption of actin filaments increases the activity of sodium-conducting channels in human myeloid leukemia cells.肌动蛋白丝的破坏会增加人髓系白血病细胞中钠传导通道的活性。
Mol Biol Cell. 1996 Dec;7(12):1857-64. doi: 10.1091/mbc.7.12.1857.
6
Regulation of calcium channel expression in neonatal myocytes by catecholamines.儿茶酚胺对新生心肌细胞钙通道表达的调节
J Clin Invest. 1996 Feb 1;97(3):656-63. doi: 10.1172/JCI118462.
7
Regulation of Ca channel by intracellular Ca2+ and Mg2+ in frog ventricular cells.蛙心室细胞中细胞内Ca2+和Mg2+对钙通道的调节
Pflugers Arch. 1996 Jan;431(3):305-17. doi: 10.1007/BF02207267.
8
Sodium-selective channels in membranes of rat macrophages.大鼠巨噬细胞膜中的钠选择性通道。
J Membr Biol. 1994 Feb;138(1):37-45. doi: 10.1007/BF00211067.
9
The P2Z-purinoceptor of human lymphocytes: actions of nucleotide agonists and irreversible inhibition by oxidized ATP.人类淋巴细胞的P2Z嘌呤受体：核苷酸激动剂的作用及被氧化ATP的不可逆抑制
Br J Pharmacol. 1994 Jul;112(3):946-50. doi: 10.1111/j.1476-5381.1994.tb13172.x.
10
Local, stochastic release of Ca2+ in voltage-clamped rat heart cells: visualization with confocal microscopy.电压钳制的大鼠心脏细胞中Ca2+的局部随机释放：共聚焦显微镜观察
J Physiol. 1994 Oct 1;480 ( Pt 1)(Pt 1):21-9. doi: 10.1113/jphysiol.1994.sp020337.
本文引用的文献
1
Inhibitory effects of sodium and other monovalent cations on human platelet adenylate cyclase.钠及其他单价阳离子对人血小板腺苷酸环化酶的抑制作用。
J Biol Chem. 1981 Oct 10;256(19):9990-3.
2
Sodium/calcium exchange in mammalian ventricular muscle: a study with sodium-sensitive micro-electrodes.哺乳动物心室肌中的钠/钙交换：一项使用钠敏感微电极的研究。
J Physiol. 1983 Oct;343:253-76. doi: 10.1113/jphysiol.1983.sp014891.
3
Beta-adrenergic modulation of calcium channels in frog ventricular heart cells.蛙心室肌细胞中钙通道的β-肾上腺素能调节
Nature. 1984;307(5949):371-5. doi: 10.1038/307371a0.
4
Ca2+ channel modulation by 8-bromocyclic AMP in cultured heart cells.8-溴环磷酸腺苷对培养心肌细胞中钙离子通道的调节作用
Nature. 1983;304(5925):462-4. doi: 10.1038/304462a0.
5
6
Calcium currents of isolated bovine ventricular myocytes are fast and of large amplitude.分离的牛心室肌细胞的钙电流快速且幅度大。
Pflugers Arch. 1982 Oct;395(1):30-41. doi: 10.1007/BF00584965.
7
Enhancement of calcium current during digitalis inotropy in mammalian heart: positive feed-back regulation by intracellular calcium?洋地黄增强哺乳动物心脏收缩力时钙电流的增强：细胞内钙的正反馈调节？
J Physiol. 1982 Aug;329:589-614. doi: 10.1113/jphysiol.1982.sp014321.
8
Properties of single calcium channels in cardiac cell culture.心脏细胞培养中单个钙通道的特性
Nature. 1982 Jun 10;297(5866):501-4. doi: 10.1038/297501a0.
9
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.用于从细胞和无细胞膜片进行高分辨率电流记录的改进膜片钳技术。
Pflugers Arch. 1981 Aug;391(2):85-100. doi: 10.1007/BF00656997.
10
Ca-dependent K channels with large unitary conductance in chromaffin cell membranes.嗜铬细胞膜中具有大的单位电导的钙依赖性钾通道。
Nature. 1981 Jun 11;291(5815):497-500. doi: 10.1038/291497a0.
Zotero 插件