Dwyer B E, Nishimura R N, De Vellis J, Yoshida T
Molecular Neurobiology Laboratory, Veterans Affairs Medical Center, Sepulveda 91343.
Glia. 1992;5(4):300-5. doi: 10.1002/glia.440050407.
Cultured rat forebrain astrocytes contained significant amounts of immunostainable heme oxygenase-1 (HO-1) isozyme, whereas HO-1 was undetectable in spontaneously transformed rat astroglial cells (ATs). HO-1 was inducible in both cell types by heat shock and by submicromolar amounts of H2O2. Inhibition of RNA synthesis with actinomycin D or protein synthesis with cycloheximide resulted in the rapid loss of immunostainable heme oxygenase in astrocytes. Analysis of the primary structure of heme oxygenase suggests that it is a PEST protein, i.e., targeted for rapid turnover.
