Gishizky M L, Witte O N
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
Science. 1992 May 8;256(5058):836-9. doi: 10.1126/science.1375394.
Expression of the bcr-abl oncogene in multipotent progenitor cells (MPPCs) is implicated as a key event in the development of chronic myelogenous leukemia. Bone marrow enriched for MPPCs was infected with a retrovirus that carried bcr-abl. The mixed-lineage colonies that resulted were responsive to growth factors and could differentiate. These cells later became growth factor-independent but, when injected into severe combined immunodeficient mice, were not leukemogenic. Thus, the presence of bcr-abl alone does not cause growth factor independence, although it initiates a stepwise process. This system may prove useful in the study of other oncogenes that cause leukemia.
多能祖细胞（MPPCs）中bcr-abl癌基因的表达被认为是慢性粒细胞白血病发生发展中的关键事件。富集了MPPCs的骨髓被携带bcr-abl的逆转录病毒感染。由此产生的混合谱系集落对生长因子有反应且能够分化。这些细胞后来变得不依赖生长因子，但当注射到严重联合免疫缺陷小鼠体内时，并不具有致白血病性。因此，单独存在bcr-abl不会导致不依赖生长因子，尽管它启动了一个逐步发展的过程。该系统可能在研究其他导致白血病的癌基因方面证明是有用的。
