Martens G J, Piosik P A, Danen E H
Department of Animal Physiology, University of Nijmegen, Toernooiveld, The Netherlands.
Biochem Biophys Res Commun. 1992 May 15;184(3):1456-9. doi: 10.1016/s0006-291x(05)80046-4.
The novel family of 14-3-3 proteins may be involved in the regulation of neuronal activity. During our search for proteins coordinately expressed with the prohormone proopiomelanocortin in the melanotrope cells of the Xenopus intermediate pituitary gland, we cloned and sequenced a pituitary cDNA encoding a Xenopus 14-3-3 protein. Alignment of the Xenopus protein with known mammalian, Drosophila and plant 14-3-3 polypeptide and with a mammalian protein kinase C inhibitor protein revealed that the neuron-specific 14-3-3-related proteins are highly conserved (60-88%) throughout eukaryotic evolution.
新发现的14-3-3蛋白家族可能参与神经元活动的调节。在我们寻找与非洲爪蟾中间脑垂体黑素细胞中阿黑皮素原前体激素协同表达的蛋白质的过程中，我们克隆并测序了一个编码非洲爪蟾14-3-3蛋白的脑垂体cDNA。将非洲爪蟾的这种蛋白与已知的哺乳动物、果蝇和植物的14-3-3多肽以及一种哺乳动物蛋白激酶C抑制蛋白进行比对后发现，在整个真核生物进化过程中，神经元特异性的14-3-3相关蛋白具有高度保守性（60%-88%）。
