促分泌素诱发的麻醉大鼠胰液分泌的时程变化

Secretagogue-evoked time-course changes on pancreatic juice secretion in the anaesthetized rat.

Salido G M, Singh J, Render C L, Camello P J

Department of Physiology, Faculty of Veterinary Sciences, University of Extremadura, Caceres, Spain.

Gen Pharmacol. 1992 Jan;23(1):33-8. doi: 10.1016/0306-3623(92)90043-j.

Abstract

In the present time-course study, we have examined the interactions between the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and the synthetic gut hormones, cholecystokinin-octapeptide (CCK-8) and secretin on pancreatic juice secretion in anaesthetized rat. 2. Administration of either TPA (10(-8) mol kg-1 hr-1), secretin (100 pmol kg-1 hr-1) or CCK-8 (150 pmol kg-1 hr-1) in the anaesthetized rat resulted in marked time-course increases in pancreatic juice flow, amylase secretion and total protein output compared to saline controls. The effect of secretin on juice flow was more pronounced and sustained compared to the smaller responses obtained with either CCK-8 or TPA. Similarly, CCK-8 evoked increases in protein output and amylase secretion compared to the responses obtained with either secretin or TPA. 3. Simultaneous infusion of TPA with either CCK-8 or secretin resulted in a marked reduction in pancreatic juice flow, total protein output and amylase secretion compared to the responses obtained with either CCK-8 or secretin alone. 4. Administration of polymyxin B (10(-8) mol kg-1 hr-1), a protein kinase C inhibitor with either TPA and CCK-8 or TPA and secretin caused a partial reduction of the inhibitory effect of TPA on CCK-8 and secretin-evoked secretory responses. 5. The present study further implicates the involvement of protein kinase C in the modulation of CCK-8 and secretin-induced pancreatic juice secretion in the anaesthetized rat.

摘要

在本时程研究中，我们检测了佛波酯12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）与合成肠道激素胆囊收缩素八肽（CCK - 8）和促胰液素对麻醉大鼠胰液分泌的相互作用。2. 在麻醉大鼠中，给予TPA（10⁻⁸ mol kg⁻¹ hr⁻¹）、促胰液素（100 pmol kg⁻¹ hr⁻¹）或CCK - 8（150 pmol kg⁻¹ hr⁻¹），与生理盐水对照组相比，胰液流量、淀粉酶分泌和总蛋白输出均呈现明显的时程性增加。与CCK - 8或TPA引起的较小反应相比，促胰液素对胰液流量的影响更显著且持续时间更长。同样，与促胰液素或TPA引起的反应相比，CCK - 8引起的蛋白输出和淀粉酶分泌增加。3. 与单独给予CCK - 8或促胰液素相比，TPA与CCK - 8或促胰液素同时输注导致胰液流量、总蛋白输出和淀粉酶分泌显著减少。4. 给予蛋白激酶C抑制剂多粘菌素B（10⁻⁸ mol kg⁻¹ hr⁻¹），与TPA和CCK - 8或TPA和促胰液素联合使用时，可部分减轻TPA对CCK - 8和促胰液素诱发的分泌反应的抑制作用。5. 本研究进一步表明蛋白激酶C参与了麻醉大鼠中CCK - 8和促胰液素诱导的胰液分泌的调节。