Camello P J, Wisdom D M, Singh J, Salido G M
Departamento de Fisiología Facultad de Veterinaria Universidad de Extremadura Cáceres, Spain.
Rev Esp Fisiol. 1994 Mar;50(1):35-40.
This study employs the isolated perfused rat pancreas to investigate the actions of the two gut hormones, cholecystokinin-octapeptide (CCK-8) and secretin on pancreatic juice flow and total protein output. Perfusion of the pancreas with either CCK-8 (100 pM) or secretin (100 pM) resulted in marked both pancreatic juice flow and total protein output. The effect of CCK-8 was three-fold larger compared to the responses obtained with secretin alone. Simultaneous application of CCK-8 and secretin (all 100 pM) failed to elicit either a potentiation or an additive effect in both pancreatic juice flow and total protein output. The net increases in both secretory parameters were reduced during combined application of CCK-8 and secretin compared to the responses obtained with CCK-8 alone. The results indicate that optimal concentrations of either CCK-8 or secretin can display marked secretagogue effects on the exocrine pancreas but when administered simultaneously they failed to elicit either an additive response or a potentiation in pancreatic juice secretion.
本研究采用离体灌注大鼠胰腺，以研究两种胃肠激素，即八肽胆囊收缩素（CCK-8）和促胰液素对胰液分泌量和总蛋白分泌量的作用。用CCK-8（100 pM）或促胰液素（100 pM）灌注胰腺，均导致胰液分泌量和总蛋白分泌量显著增加。与单独使用促胰液素所获得的反应相比，CCK-8的作用大三倍。同时应用CCK-8和促胰液素（均为100 pM），在胰液分泌量和总蛋白分泌量方面均未引起增强效应或相加效应。与单独使用CCK-8所获得的反应相比，联合应用CCK-8和促胰液素时，两种分泌参数的净增加量均减少。结果表明，CCK-8或促胰液素的最佳浓度均可对胰腺外分泌产生显著的促分泌作用，但同时给药时，它们在胰液分泌中未能引起相加反应或增强效应。
