Ogden G R, Nairn A, Carmichael A, Coghill G, Cree I A, Green M W, Hopwood D V, Chisholm D M
Department of Dental Surgery, University of Dundee, Scotland.
J Oral Pathol Med. 1992 Jan;21(1):17-20. doi: 10.1111/j.1600-0714.1992.tb00962.x.
Cellular expression of keratins (intermediate filaments) can be demonstrated by immunocytochemistry using unfixed tissue. However, for practical reasons, provision of fresh tissue to the laboratory is often difficult. Recently a fresh tissue transport medium (Patent applied for) has been developed which allows keratin immunocytochemistry to be performed up to 4 days after biopsy. In this study oral mucosal biopsies from 10 patients were hemisected, half placed in the new transport medium at 4 degrees C and the other half immediately frozen in liquid nitrogen. After 4 days frozen sections of both halves of the biopsies were stained by immunocytochemistry using various antikeratin antibodies. The morphology and staining characteristics of the two halves of the biopsies were then assessed. No significant difference could be found in either morphology or preservation of keratin expression in specimens stored in transport medium, as compared to those in liquid nitrogen. This new transport medium may offer considerable advantage for the provision of a histologic and immunocytochemical diagnostic service.
角蛋白（中间丝）的细胞表达可以通过使用未固定组织的免疫细胞化学来证明。然而，出于实际原因，向实验室提供新鲜组织往往很困难。最近开发了一种新鲜组织运输培养基（已申请专利），它可以在活检后4天内进行角蛋白免疫细胞化学检测。在本研究中，将10名患者的口腔黏膜活检标本切成两半，一半置于4℃的新运输培养基中，另一半立即在液氮中冷冻。4天后，对活检标本两半的冰冻切片使用各种抗角蛋白抗体进行免疫细胞化学染色。然后评估活检标本两半的形态和染色特征。与液氮保存的标本相比，运输培养基中保存的标本在形态或角蛋白表达保存方面均未发现显著差异。这种新的运输培养基可能为提供组织学和免疫细胞化学诊断服务带来相当大的优势。
