Heyden A, Huitfeldt H S, Koppang H S, Thrane P S, Bryne M, Brandtzaeg P
Institute of Pathology, Medical Faculty, National Hospital, Oslo, Norway.
J Oral Pathol Med. 1992 Jan;21(1):7-11. doi: 10.1111/j.1600-0714.1992.tb00960.x.
The keratin expression pattern in oral stratified epithelium is related to the cellular differentiation level. The normal pattern shows the keratin pair K5 and K14 in the stratum basale whereas K1 and K10, or K4 and K13, are the two pairs associated with differentiating suprabasal cells. Monoclonal and polyclonal antibodies to individual keratins (K10, K13 and K14) were used in a two-color immunofluorescence staining method to study their coexpression in single cells. Altered keratin expression in premalignant and malignant lesions indicated abnormal differentiation. Monospecific keratin antibodies were suggested to be useful for evaluation of epithelial differentiation changes in oral dysplasias and oral squamous cell carcinomas.
口腔复层上皮中的角蛋白表达模式与细胞分化水平相关。正常模式显示，基底细胞层中有角蛋白对K5和K14，而K1和K10或K4和K13是与基底上层分化细胞相关的两对角蛋白。采用针对单个角蛋白（K10、K13和K14）的单克隆和多克隆抗体，通过双色免疫荧光染色法研究它们在单个细胞中的共表达情况。癌前病变和恶性病变中角蛋白表达的改变表明分化异常。单特异性角蛋白抗体被认为有助于评估口腔发育异常和口腔鳞状细胞癌中的上皮分化变化。
