Teixeira N A, O'Grady J E
Faculdade de Farmácia, Universidade do Porto, Portugal.
J Reprod Fertil. 1992 Mar;94(2):501-9. doi: 10.1530/jrf.0.0940501.
After isolation and purification, rat acute-phase protein, alphamacrofetoprotein and the uterine decidual protein, decidualization-associated protein were compared. They are similar in molecular weight and amino acid composition, and behave similarly during native polyacrylamide gel electrophoresis and are crossreactive with polyclonal antisera. They differ in pI values: with a mean pI of 4.83 for alphamacrofetoprotein, 5.16 for the major form of decidualization-associated protein and 4.97 for the remainder. Alphamacrofetoprotein and decidualization-associated protein differ in carbohydrate content and subunit structure, but show similar susceptibility to trypsin digestion. Evidence is provided that both the decidual species form complexes with proteases and that these are present in the extracted decidual proteins. The distribution of the forms of decidualization-associated protein in the tissues during pregnancy and parturition is described and discussed.
经过分离和纯化后，对大鼠急性期蛋白、甲胎蛋白以及子宫蜕膜蛋白（蜕膜化相关蛋白）进行了比较。它们在分子量和氨基酸组成上相似，在天然聚丙烯酰胺凝胶电泳过程中的表现相似，并且与多克隆抗血清有交叉反应。它们的等电点值不同：甲胎蛋白的平均等电点为4.83，蜕膜化相关蛋白主要形式的平均等电点为5.16，其余部分的平均等电点为4.97。甲胎蛋白和蜕膜化相关蛋白在碳水化合物含量和亚基结构上有所不同，但对胰蛋白酶消化的敏感性相似。有证据表明，这两种蜕膜蛋白都与蛋白酶形成复合物，并且这些复合物存在于提取的蜕膜蛋白中。本文描述并讨论了蜕膜化相关蛋白在妊娠和分娩期间在组织中的分布形式。
