Rat alpha2 acute-phase macroglobulin was isolated from turpentine-injected rats by Sephadex G-200 chromatography and ion-exchange chromatography on DEAE-cellulose. This method, since it does not include (NH4)2SO4 treatment, allows the study of the physicochemical as well as the biological properties of the molecule. 2. The purity of the preparation was demonstrated by ultracentrifugation, polyacrylamide-gel electrophoresis, fused "rocket" immunoelectrophoresis as well as double immunodiffusion. 3. The rat alpha2 acute-phase macroglobulin was characterized in terms of its main physical and chemical properties. Its isoelctric point was determined by isoelectrofocusing to be 4.55; s020,w was 18.4S and E1%/1cm at 278 nm was 6.8. The mol.wt. was determined by light-scattering to be 770000. 4. The amino acid content was compared with that of rat alpha1 macroglobulin and was found very similar. The carbohydrate composition of alpha2 acute-phase macroglobulin was determined to be: hexose, 4.25%; glucosamine, 3.4%; sialic acid, 2%; fucose, 0.2%. From these results it was concluded that alpha2 acute-phase macroglobulin, although a typical acute-phase reactant, possesses the characteristic physicochemical properties of alpha macroglobulins.
摘要
通过Sephadex G - 200凝胶过滤色谱法和DEAE - 纤维素离子交换色谱法，从注射松节油的大鼠中分离出大鼠α2急性期巨球蛋白。该方法由于不包括硫酸铵处理，因此可以研究该分子的物理化学性质以及生物学性质。2. 通过超速离心、聚丙烯酰胺凝胶电泳、融合“火箭”免疫电泳以及双向免疫扩散证明了制剂的纯度。3. 对大鼠α2急性期巨球蛋白的主要物理化学性质进行了表征。通过等电聚焦测定其等电点为4.55；s020,w为18.4S，在278nm处的E1%/1cm为6.8。通过光散射测定其分子量为770000。4. 将氨基酸含量与大鼠α1巨球蛋白的氨基酸含量进行比较，发现非常相似。测定α2急性期巨球蛋白的碳水化合物组成如下：己糖，4.25%；氨基葡萄糖，3.4%；唾液酸，2%；岩藻糖，0.2%。从这些结果可以得出结论，α2急性期巨球蛋白虽然是一种典型的急性期反应物，但具有α巨球蛋白的特征性物理化学性质。
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