KLECZKOWSKI A
Immunology. 1961 Apr;4(2):130-41.
The protein obtained when tobacco mosaic virus is disrupted with alkali is antigenically heterogeneous, whereas the intact virus is homogeneous. Aggregating the protein by acidification makes it again antigenically homogeneous. In comparable conditions, antibody/antigen ratio is considerably higher in precipitates formed by the protein than in those formed by the virus. Aggregating the protein restores the ratio to that given by the original virus. Electron-microscopic examination shows that in extreme antibody excess each virus particle is covered with a layer of antibody molecules. In these conditions, therefore, individual antibody molecules seem not to bridge directly between different virus particles, but they may do so in the zone of equivalence.
用碱破坏烟草花叶病毒时所获得的蛋白质在抗原性上是异质的，而完整的病毒则是同质的。通过酸化使该蛋白质聚集会使其在抗原性上再次变得同质。在可比条件下，由该蛋白质形成的沉淀物中的抗体/抗原比率比由病毒形成的沉淀物中的比率要高得多。使该蛋白质聚集可将该比率恢复到原始病毒所给出的比率。电子显微镜检查表明，在抗体严重过量的情况下，每个病毒颗粒都被一层抗体分子覆盖。因此，在这些条件下，单个抗体分子似乎不会直接在不同病毒颗粒之间形成桥连，但它们可能在等价区这样做。
