Butchaiah G, Card J L, Morgan D O
Southern Regional Station, Indian Veterinary Research Institute, Bangalore.
Vet Immunol Immunopathol. 1992 Jan 15;30(2-3):275-92. doi: 10.1016/0165-2427(92)90144-f.
A panel (26) of monoclonal antibodies (MAbs) was elicited against three distinct isolates of foot-and-mouth disease virus (FMDV) serotype Asia-1. Each MAb was characterized according to the location of its epitope: Class I, restricted to the intact virion (140S); Class II, restricted to 140S and the virion protein subunit (12Sps); Class III, available on 140S, 12Sps and virus protein 1; Class IV, restricted to 12Sps. In addition, the MAbs were further categorized by isotype, neutralization of viral infectivity, capacity to bind in radioimmunoassay and precipitation in the Ouchterlony reaction. Neutralization of FMDV infectivity by a MAb of the IgA isotype is reported for the first time. A minimum of seven distinct neutralization epitopes were described on FMDV Asia-1. Some of the neutralizing MAbs bound FMDVs in addition to those that they neutralized. The MAbs defined epitopes common to FMDV serotypes Asia-1, A, O1 and C but neutralizing capacity was restricted to serotype Asia-1. Class IV MAbs defined epitopes highly conserved throughout the FMDV serotypes. Identification of FMDV neutralization epitopes makes possible the direct selection of optimal FMDV strains for vaccine fabrication. In addition, these data are crucial to the design of future synthetic vaccines.
制备了一组（26种）针对口蹄疫病毒（FMDV）亚洲1型三种不同分离株的单克隆抗体（MAb）。每种单克隆抗体根据其表位的位置进行了表征：I类，仅限于完整病毒粒子（140S）；II类，仅限于140S和病毒粒子蛋白亚基（12Sps）；III类，在140S、12Sps和病毒蛋白1上均可获得；IV类，仅限于12Sps。此外，这些单克隆抗体还根据同种型、病毒感染性的中和作用、放射免疫测定中的结合能力以及免疫双扩散反应中的沉淀作用进一步分类。首次报道了IgA同种型单克隆抗体对FMDV感染性的中和作用。在FMDV亚洲1型上描述了至少七个不同的中和表位。一些中和性单克隆抗体除了能中和的FMDV外，还能结合其他FMDV。这些单克隆抗体确定了FMDV亚洲1型、A、O1和C血清型共有的表位，但中和能力仅限于亚洲1型血清型。IV类单克隆抗体确定了在整个FMDV血清型中高度保守的表位。FMDV中和表位的鉴定使得直接选择用于疫苗制备的最佳FMDV毒株成为可能。此外，这些数据对于未来合成疫苗的设计至关重要。
