通过硫酸铵沉淀法制备人免疫球蛋白。
Preparation of human immunoglobulin by ammonium sulfate precipitation.
作者信息
Habeeb A F, Francis R D
出版信息
Vox Sang. 1976;31(6):423-34. doi: 10.1111/j.1423-0410.1976.tb04457.x.
Abstract
Crude IgG precipitated from plasma by the addition of an equal volume of 4 M ammonium sulfate was fractionated on DEAE-cellulose column to obtain pure IgG as shown by disc electrophoresis, immunoelectrophoresis and gel filtration. The processed IgG was free of plasmin and plasminogen and did not undergo fragmentation or aggregation on storage at 40 degrees C for at least 4 weeks. The anticomplement activity was low and the antibody activity was retained.
摘要
通过加入等体积的4M硫酸铵从血浆中沉淀出的粗制IgG，在DEAE-纤维素柱上进行分级分离，以获得如圆盘电泳、免疫电泳和凝胶过滤所示的纯IgG。处理后的IgG不含纤溶酶和纤溶酶原，在40℃储存至少4周时不会发生片段化或聚集。其抗补体活性较低且抗体活性得以保留。
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