血清蛋白分级分离研究。

A study of serum protein fractionation.

出版信息

Am J Med Technol. 1979 Jan;45(1):11-5.

Abstract

Serum protein fractionation was studied using the techniques of ammonium sulfate precipitation, molecular seiving chromatography, and ion-exchange chromatography. Whole human serum was fractionated by precipitation with ammonium sulfate at 40, 50, 60, and 70 percent saturation. Molecular seiving chromatography was performed on whole human serum and on four ammonium sulfate precipitated fractions. The eluted peaks from molecular seiving chromatography were further separated by ion-exchange chromatography to obtain isolated fractions of IgG, transferrin, and albumin. Qualitative analysis of the various fractions and assessment of the purity of isolated IgG, transferrin, and albumin was done by polyacrylamide gel electrophoresis and by immunoelectrophoresis.

摘要

采用硫酸铵沉淀、分子筛色谱和离子交换色谱技术研究了血清蛋白分级分离。通过用饱和度为40%、50%、60%和70%的硫酸铵沉淀对全人血清进行分级分离。对全人血清和四个硫酸铵沉淀级分进行了分子筛色谱分析。分子筛色谱洗脱峰通过离子交换色谱进一步分离，以获得纯化的IgG、转铁蛋白和白蛋白级分。通过聚丙烯酰胺凝胶电泳和免疫电泳对各个级分进行定性分析，并评估纯化的IgG、转铁蛋白和白蛋白的纯度。