Kunnas T A, Joensuu T K, Viitala K K, Sopanen P, Tuohimaa P, Kulomaa M S
University of Tampere, Department of Biomedical Sciences, Finland.
Endocrinology. 1992 Jun;130(6):3421-6. doi: 10.1210/endo.130.6.1375902.
Avidin gene expression was analyzed using an avidin immunoassay and RNA hybridization analysis. To ascertain whether the induction of the avidin gene by progesterone remains specific also during secondary restimulation with diethylstilbestrol, chicks were given different steroid hormones or hormone combinations. Progesterone-specific induction of avidin protein and messenger RNA (mRNA) was 15- to 30-fold over the control even after secondary restimulation with diethylstilbestrol. A functional difference between the progesterone response element and glucocorticoid response element was suggested, since dexamethasone alone did not induce avidin in vivo. In spite of progesterone specificity, a combination of progesterone with other steroids nevertheless generated a synergistic increase in the amount of avidin mRNA. This may indicate that binding of progesterone receptor to the progesterone response element may be important to alter the functional activity of other hormone response elements present on the avidin gene. The time response curve of the avidin mRNA induction by progesterone was also determined. Avidin mRNA was detectable 8 h after progesterone induction, and its amount was maximal after 16-24 h. This would indicate that the avidin gene belongs in the so-called late responder genes, which also include chicken ovalbumin, ovomucoid, and lysozyme genes.
使用抗生物素蛋白免疫测定法和RNA杂交分析对抗生物素蛋白基因表达进行了分析。为了确定在用己烯雌酚进行二次再刺激期间，孕酮对抗生物素蛋白基因的诱导是否仍然具有特异性，给雏鸡给予了不同的类固醇激素或激素组合。即使在用己烯雌酚进行二次再刺激后，孕酮对抗生物素蛋白和信使核糖核酸（mRNA）的特异性诱导仍比对照高15至30倍。由于单独的地塞米松在体内不诱导抗生物素蛋白，提示孕酮反应元件和糖皮质激素反应元件之间存在功能差异。尽管具有孕酮特异性，但孕酮与其他类固醇的组合仍使抗生物素蛋白mRNA的量产生协同增加。这可能表明孕酮受体与孕酮反应元件的结合对于改变抗生物素蛋白基因上存在的其他激素反应元件的功能活性可能很重要。还测定了孕酮诱导抗生物素蛋白mRNA的时间反应曲线。孕酮诱导后8小时可检测到抗生物素蛋白mRNA，其含量在16至24小时后达到最大值。这表明抗生物素蛋白基因属于所谓的晚期反应基因，其中还包括鸡卵清蛋白、卵类粘蛋白和溶菌酶基因。
