Arao Y, Miyatake N, Yamamoto E, Usiku H, Matsuura A, Ninomiya Y, Masushige S, Hasegawa T, Kato S
Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture.
J Biochem. 1996 Oct;120(4):710-5. doi: 10.1093/oxfordjournals.jbchem.a021469.
The stabilization of chicken ovalbumin (OVA) mRNA by different classes of steroid hormones (estrogen, progesterone, glucocorticoid, and androgen) was studied in the oviducts of chicks treated with combinations of four steroids. The combination of estrogen with progesterone, glucocorticoid, or androgen enhanced the induction of the OVA gene more than did estrogen alone. Run-on analysis of the isolated oviduct nuclei to measure the transcription rate of the OVA gene showed that the enhanced induction of the OVA gene by the combined hormone treatments was partly caused by an increased rate of transcription. The half-life of OVA mRNA as determined using a transcription inhibitor (actinomycin D) was estimated to be about 24 h irrespective of the hormone treatment, though the half-life was about 6 h in the absence of hormones. These results suggested that the prolongation of the half-life of OVA mRNA by steroid hormones is constant irrespective of differential transcription rates of the OVA gene.
在接受四种类固醇激素组合处理的雏鸡输卵管中，研究了不同种类的类固醇激素（雌激素、孕酮、糖皮质激素和雄激素）对鸡卵清蛋白（OVA）mRNA的稳定性的影响。雌激素与孕酮、糖皮质激素或雄激素联合使用时，比单独使用雌激素更能增强OVA基因的诱导表达。对分离出的输卵管细胞核进行连续分析以测量OVA基因的转录速率，结果表明，联合激素处理增强OVA基因诱导表达的部分原因是转录速率增加。使用转录抑制剂放线菌素D测定的OVA mRNA半衰期估计约为24小时，与激素处理无关，尽管在无激素情况下半衰期约为6小时。这些结果表明，类固醇激素延长OVA mRNA半衰期的作用是恒定的，与OVA基因不同的转录速率无关。
