Erkman L, Mattenberger L, Kato A C
Division of Clinical Neurophysiology, Centre Médical Universitaire, Geneva, Switzerland.
Brain Res Dev Brain Res. 1992 Mar 20;66(1):109-17. doi: 10.1016/0165-3806(92)90146-n.
Monoclonal antibodies were prepared by using the anterior horn region of human embryonic spinal cord as immunogen. To increase the specificity of the immune response towards the anterior horn cells, mice were first injected with antigens from the posterior horn and then immunosuppressed with cyclophosphamide; subsequently antigens from the anterior horn were injected. One of the monoclonal antibodies recognizes a small population of anterior horn cells of human embryonic spinal cord during a transient period of development (9-10th embryonic week); these cells are probably motoneurons according to their location in the spinal cord, their positive staining for acetylcholinesterase and their large nuclei. The staining pattern has a special axial distribution as it is limited to the cervical and thoracic regions of the spinal cord. The antibody is species-specific and shows a high degree of tissue specificity. Since this antibody distinguishes a small group of anterior horn cells in the spinal cord during a specific developmental stage, it opens stimulating perspectives for further investigation on the nature of the antigen and its putative role during the development of the human embryonic spinal cord.
以人胚胎脊髓前角区域作为免疫原制备单克隆抗体。为提高对前角细胞免疫反应的特异性，先给小鼠注射后角抗原，然后用环磷酰胺进行免疫抑制；随后注射前角抗原。其中一种单克隆抗体可识别在发育的一个短暂时期（胚胎第9 - 10周）人胚胎脊髓中的一小群前角细胞；根据这些细胞在脊髓中的位置、乙酰胆碱酯酶阳性染色以及大细胞核，它们可能是运动神经元。染色模式具有特殊的轴向分布，因为它仅限于脊髓的颈段和胸段区域。该抗体具有种属特异性，并表现出高度的组织特异性。由于这种抗体能在特定发育阶段区分脊髓中的一小群前角细胞，它为进一步研究该抗原的性质及其在人胚胎脊髓发育过程中的假定作用开辟了令人兴奋的前景。
