Bosse E, Bottlender R, Kleppisch T, Hescheler J, Welling A, Hofmann F, Flockerzi V
Institut für Pharmakologie und Toxikologie, Technische Universität München, FRG.
EMBO J. 1992 Jun;11(6):2033-8. doi: 10.1002/j.1460-2075.1992.tb05260.x.
Voltage-activated calcium channels are membrane spanning proteins that allow the controlled entry of Ca2+ into the cytoplasm of cells. The principal channel forming subunit of an L-type calcium channel is the alpha 1 subunit. Transfection of Chinese hamster ovary (CHO) cells with complementary DNA encoding the calcium channel alpha 1 subunit from smooth muscle led to the expression of functional calcium channels which bind calcium channel blockers and show the voltage-dependent activation and slow inactivation and unitary current conductance characteristic of calcium channels in smooth muscle. The currents mediated by these channels are sensitive towards dihydropyridine-type blockers and agonists indicating that the calcium channel blocker receptor sites were present in functional form. The smooth muscle alpha 1 subunit cDNA alone is sufficient for stable expression of functional calcium channels with the expected kinetic and pharmacological properties in mammalian somatic cells.
电压门控性钙通道是跨膜蛋白，可使Ca2+ 可控地进入细胞胞质。L型钙通道的主要通道形成亚基是α1亚基。用编码平滑肌钙通道α1亚基的互补DNA转染中国仓鼠卵巢（CHO）细胞，导致功能性钙通道的表达，这些通道可结合钙通道阻滞剂，并表现出电压依赖性激活、缓慢失活以及平滑肌钙通道的单通道电流电导特性。由这些通道介导的电流对二氢吡啶类阻滞剂和激动剂敏感，表明钙通道阻滞剂受体位点以功能形式存在。单独的平滑肌α1亚基cDNA足以在哺乳动物体细胞中稳定表达具有预期动力学和药理学特性的功能性钙通道。
