人粒细胞-巨噬细胞集落刺激因子：互补DNA的分子克隆以及天然和重组蛋白的纯化。

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.

作者信息

Wong G G, Witek J S, Temple P A, Wilkens K M, Leary A C, Luxenberg D P, Jones S S, Brown E L, Kay R M, Orr E C

出版信息

Science. 1985 May 17;228(4701):810-5. doi: 10.1126/science.3923623.

Abstract

Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.

摘要

通过哺乳动物细胞（猴COS细胞）表达筛选系统分离出编码称为粒细胞-巨噬细胞集落刺激因子（GM-CSF）的人淋巴因子的互补DNA克隆。其中一个克隆用于在哺乳动物细胞中产生重组GM-CSF。该重组造血蛋白与从人T细胞系条件培养基中纯化至表观均一性的天然产物相似。发现人T细胞GM-CSF与最近从鼠肺信使RNA克隆的GM-CSF有60%的同源性。