Vankova M, Arluison M, Leviel V, Tramu G
Medical Academy, Dept Anatomy, Sofia, Bulgaria.
J Chem Neuroanat. 1992 Jan-Feb;5(1):39-50. doi: 10.1016/0891-0618(92)90032-l.
The afferent connections of the rat substantia nigra pars lateralis have been studied using the retrograde axonal transport of fluorescent latex microspheres. The most numerous groups of retrogradely labelled nerve cell bodies were observed bilaterally in the parabrachial complex and several hypothalamic nuclei, whereas the parietal neocortex, the fundus striati, the central nucleus of the amygdala and the bed nucleus of the stria terminalis were labelled on the injected side only. The neuronal projections from the central amygdaloid nucleus to the substantia nigra pars lateralis and lateral part of the rostral pars compacta have additionally been confirmed by anterograde tracing using wheat-germ agglutinin coupled to horseradish peroxidase. The presence of some peptides in this pathway was studied by combining the use of the same retrograde tracer with immunofluorescence after intra-amygdaloid injections of colchicine. With this method, we have demonstrated that Met-enkephalin, dynorphin and neurotensin are probably utilized as neurotransmitters or co-transmitters in the neurons of the amygdalo-nigral pathway.
利用荧光乳胶微球的逆行轴突运输，对大鼠黑质外侧部的传入连接进行了研究。在双侧臂旁复合体和几个下丘脑核中观察到数量最多的逆行标记神经细胞体群，而顶叶新皮层、纹状体底部、杏仁核中央核和终纹床核仅在注射侧有标记。通过使用与辣根过氧化物酶偶联的麦胚凝集素进行顺行追踪，进一步证实了从杏仁核中央核到黑质外侧部和致密部前部外侧的神经元投射。通过在杏仁核内注射秋水仙碱后，将相同的逆行示踪剂与免疫荧光结合使用，研究了该通路中某些肽的存在情况。用这种方法，我们已经证明甲硫氨酸脑啡肽、强啡肽和神经降压素可能在杏仁核 - 黑质通路的神经元中用作神经递质或共递质。
