Arluison M, Brochier G, Vankova M, Leviel V, Villalobos J, Tramu G
Lab. de Neurobiologie des Signaux Intercellulaires, Institut des Neurosciences CNRS, Université P. et M. Curie, Paris, France.
Brain Res Bull. 1994;34(4):319-37. doi: 10.1016/0361-9230(94)90026-4.
In the present study, we demonstrate the existence of numerous peptidergic afferents to the bed nucleus of the stria terminalis (BNST) using the retrograde transport of gold-labeled wheat germ agglutinin-apo-peroxidase (G-WGA-HRP) combined with the indirect immunoperoxidase method after intraparenchymatous injections of colchicine. At first, we show that local injections of colchicine alone into the BNST are able to induce the retrograde accumulation of peptides until the nerve cell bodies of origin, probably because of the blockade of axonal transport in nerve terminal arborizations innervating this nucleus. The actual existence of putative peptidergic afferents to the BNST indicated by the local injections of colchicine was established using: a) the retrograde transport of G-WGA-HRP from the BNST combined with immunocytochemistry after administration of colchicine at the same place, b) the anterograde "transport" of the fluorescent tracer DiI from selected nuclei of the forebrain. We demonstrate that the neurons immunoreactive for enkephalins, neurotensin, or substance P that innervate the BNST are localized mainly in the central amygdaloid nucleus, the paraventricular thalamic nucleus, and the ventromedial hypothalamic nucleus ipsilateral to the injection, as well as bilaterally in the magnocellular paraventricular and perifornical regions of the hypothalamus. From these results it may be concluded that intracerebral injections of colchicine constitute a powerful tool to search for multiple peptidergic afferents to a given brain nucleus using only immunohistochemistry. The existence of these pathways, however, must be verified by other neuroanatomical methods because of the problem of nerve fibers of passage.
在本研究中，我们通过在脑实质内注射秋水仙碱后，利用金标记的小麦胚凝集素 - 脱辅基过氧化物酶（G - WGA - HRP）的逆行运输结合间接免疫过氧化物酶法，证明了存在大量向终纹床核（BNST）投射的肽能传入纤维。首先，我们表明单独向BNST局部注射秋水仙碱能够诱导肽类逆行积聚直至其起源的神经细胞体，这可能是由于支配该核的神经末梢分支中的轴突运输受阻所致。通过以下方法证实了局部注射秋水仙碱所提示的向BNST投射的假定肽能传入纤维的实际存在：a）在同一部位注射秋水仙碱后，结合免疫细胞化学技术，利用G - WGA - HRP从BNST进行逆行运输；b）从前脑的选定核团进行荧光示踪剂DiI的顺行“运输”。我们证明，支配BNST的脑啡肽、神经降压素或P物质免疫反应性神经元主要位于注射侧的中央杏仁核、丘脑室旁核和下丘脑腹内侧核，以及双侧下丘脑的大细胞室旁区和穹窿周区。从这些结果可以得出结论，脑内注射秋水仙碱构成了一种强大的工具，仅使用免疫组织化学方法就可以寻找向给定脑核投射的多种肽能传入纤维。然而，由于存在过路神经纤维的问题，这些通路的存在必须通过其他神经解剖学方法进行验证。
