Sim T C, Alam R, Forsythe P A, Welter J B, Lett-Brown M A, Grant J A
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550-2780.
J Allergy Clin Immunol. 1992 Jun;89(6):1157-65. doi: 10.1016/0091-6749(92)90300-q.
We collected individual pools of nasal washings (NWs) from 15 allergic and 15 nonallergic subjects to determine histamine-releasing factor (HRF) activity and to ascertain the relationship of these cytokines with atopic status, basophil releasability, and cell membrane-bound IgE. NWs were concentrated, dialyzed, and assayed with basophils from a single donor. Samples from 12 of 15 allergic subjects and from all the nonallergic subjects revealed greater than or equal to 15% histamine release (HR), 33.5% +/- 21.3% (mean +/- SD) and 38.6% +/- 19.6%, respectively (p greater than 0.05). When we assayed the same samples with autologous basophils, the allergic group demonstrated higher HR than the nonallergic group (31.9% +/- 19.7% versus 4.8% +/- 4.3%; p less than 0.001). A standard lot of mononuclear cell-derived HRFs was also screened with basophils from both groups. Means for HR from basophils of allergic and nonallergic subjects were 51.9% +/- 16.7% versus 26.3% +/- 8.2%, respectively (p less than 0.001). Pretreatment of basophils with lactic acid led to abrogation of sensitivity to HRF. Acid-stripped cells incubated with sera from patients with asthma regained their capacity to release histamine. We found that HRF activity can be detected in NWs of most donors, and there is no difference among allergic and nonallergic subjects. Our results suggest that the capacity of these cytokines to induce HR depends on several factors: atopic status, basophil releasability, and membrane-bound IgE.
我们收集了15名过敏受试者和15名非过敏受试者的个体鼻腔冲洗液样本，以测定组胺释放因子（HRF）活性，并确定这些细胞因子与特应性状态、嗜碱性粒细胞释放能力及细胞膜结合IgE之间的关系。将鼻腔冲洗液浓缩、透析，并用来自单一供体的嗜碱性粒细胞进行检测。15名过敏受试者中的12名以及所有非过敏受试者的样本显示组胺释放（HR）大于或等于15%，分别为33.5%±21.3%（平均值±标准差）和38.6%±19.6%（p>0.05）。当我们用自体嗜碱性粒细胞检测相同样本时，过敏组的组胺释放高于非过敏组（31.9%±19.7%对4.8%±4.3%；p<0.001）。还用两组的嗜碱性粒细胞对一批标准的单核细胞衍生HRF进行了检测。过敏和非过敏受试者嗜碱性粒细胞的组胺释放平均值分别为51.9%±16.7%和26.3%±8.2%（p<0.001）。用乳酸预处理嗜碱性粒细胞可消除其对HRF的敏感性。用哮喘患者血清孵育酸剥离细胞后，其组胺释放能力得以恢复。我们发现大多数供体的鼻腔冲洗液中均可检测到HRF活性，过敏和非过敏受试者之间无差异。我们的结果表明，这些细胞因子诱导组胺释放的能力取决于几个因素：特应性状态、嗜碱性粒细胞释放能力及膜结合IgE。
