Brolin J, Skoog L, Ekman P
Department of Urology, Karolinska Hospital, Stockholm, Sweden.
Prostate. 1992;20(4):281-95. doi: 10.1002/pros.2990200404.
The relative distribution of androgen (AR), progesterone (PR), and estrogen receptors (ER) was localized and estimated in human prostate tissue by immunohistochemistry in five normal tissue samples, in eight benign hyperplastic (BPH) samples, in nine primary cancers, and in seven prostate cancer metastases. Moreover, three prostatic cancer cell lines (LNCaP, DU 145, and PC 3) were analyzed. A comparison between the results obtained by radioligand binding assays and immunohistochemistry was performed for the AR and PR. Using immunohistochemistry, the AR was exclusively detected in the nuclei of both benign and malignant prostatic epithelial cells. The highest proportion of AR-positive cells was found in BPH and in prostate cancer metastases as compared with normal prostatic tissue. In a majority of the cases, the PR was only present in the nuclei of stromal cells. Benign hyperplastic prostates contained higher proportions of PR-positive cells as compared with primary carcinoma. PR was sparse in epithelial cells. ER-positive stromal cell nuclei were only detected in carcinomatous prostates. A few ER-positive epithelial cell nuclei were found in one sample each of a BPH and normal prostate. All cells from the androgen-dependent, LNCaP, cell line and a majority of the cells from the androgen-independent, DU 145, cell line were AR-positive. In contrast, the cells from the androgen-independent, PC 3, cell line were all AR-negative. All three cell lines were PR- and ER-negative. The radioligand binding technique detected the AR in extracts from both the cytosol and the nucleus. Again BPH contained higher amounts of AR as compared with normal prostatic tissue. The LNCaP cells contained high amounts of cytosolic AR while cells from the DU 145 and PC 3 cell lines lacked detectable AR as estimated by biochemical techniques. There seemed to be a discrepancy between biochemically measured and immunohistochemically estimated receptor content.
通过免疫组织化学方法，在5份正常组织样本、8份良性增生（BPH）样本、9份原发性癌样本和7份前列腺癌转移样本中，对雄激素（AR）、孕激素（PR）和雌激素受体（ER）的相对分布进行了定位和评估。此外，还分析了三种前列腺癌细胞系（LNCaP、DU 145和PC 3）。对AR和PR进行了放射性配体结合试验结果与免疫组织化学结果的比较。使用免疫组织化学方法，仅在良性和恶性前列腺上皮细胞的细胞核中检测到AR。与正常前列腺组织相比，BPH和前列腺癌转移样本中AR阳性细胞的比例最高。在大多数情况下，PR仅存在于基质细胞的细胞核中。与原发性癌相比，良性增生的前列腺中PR阳性细胞的比例更高。PR在上皮细胞中稀少。仅在癌性前列腺中检测到ER阳性的基质细胞核。在一份BPH样本和一份正常前列腺样本中各发现了一些ER阳性的上皮细胞核。雄激素依赖性LNCaP细胞系的所有细胞以及雄激素非依赖性DU 145细胞系的大多数细胞均为AR阳性。相反，雄激素非依赖性PC 3细胞系的细胞均为AR阴性。所有三种细胞系均为PR和ER阴性。放射性配体结合技术在细胞质和细胞核提取物中均检测到了AR。同样，与正常前列腺组织相比，BPH中AR的含量更高。LNCaP细胞含有大量的细胞质AR，而根据生化技术估计，DU 145和PC 3细胞系的细胞缺乏可检测到的AR。生化测量的受体含量与免疫组织化学估计的受体含量之间似乎存在差异。
