MOOR H, MUHLETHALER K, WALDNER H, FREY-WYSSLING A
J Biophys Biochem Cytol. 1961 May;10(1):1-13. doi: 10.1083/jcb.10.1.1.
The difficulties in sectioning frozen biological objects for electron microscopic investigations are overcome by Steere's freezing-etching method. In order to test this method and to open up a wide field of application, the new freezing-ultramicrotome has been designed. The apparatus consists of the combination of an ultramicrotome with freezing-drying and shadow-casting installations in the same vacuum container. The preliminary results show, on the one hand, the practicability of all preparational steps and, on the other, that it is possible to resolve internal structures of cell organelles and even macromolecular patterns.
斯特尔的冷冻蚀刻法克服了为电子显微镜研究对冷冻生物样本进行切片时所遇到的困难。为了测试该方法并开拓广阔的应用领域,人们设计了新型冷冻超薄切片机。该设备由一台超薄切片机与冷冻干燥及投影装置组合而成,它们都置于同一个真空容器中。初步结果表明,一方面,所有制备步骤切实可行;另一方面,解析细胞器的内部结构乃至大分子模式是可能的。