Sjöström M, Squire J M
J Microsc. 1977 Dec;111(3):239-78. doi: 10.1111/j.1365-2818.1977.tb00067.x.
In the past, the techniques of electron microscopy and X-ray diffraction have both been very informative about the ultrastructure of the muscle myofibril But X-ray diffraction patterns are difficult to interpret unambiguously and until now specimen preservation in plastic embedded muscle has been sufficiently poor to make it difficult to use electron micrographs of muscle as a means of interpreting the available X-ray diffraction evidence. The possibility of using ultrathin sections of frozen muscle, in which the disruptive steps of chemical dehydration and plastic embedding can be avoided, promises to help to bridge the information gap between present X-ray and electron microscope results. For this reason we here review the application of the cryosectioning technique to muscle, we assess the technique in terms of the improvements in preservation which have so far been obtained and which might be expected and we discuss some of the many potential advantages and uses of this technique for studies of muscle ultrastructure and function. It is concluded that this technique should be developed vigorously since it promises to play a very important role in muscle research in the future.
过去，电子显微镜技术和X射线衍射技术在揭示肌肉肌原纤维的超微结构方面都提供了丰富的信息。但是X射线衍射图谱难以进行明确的解读，而且直到现在，塑料包埋肌肉中的标本保存状况一直很差，以至于难以将肌肉的电子显微镜照片作为解释现有X射线衍射证据的一种手段。使用冷冻肌肉的超薄切片有望帮助弥合当前X射线和电子显微镜结果之间的信息差距，因为在冷冻肌肉的超薄切片中可以避免化学脱水和塑料包埋这些破坏性步骤。出于这个原因，我们在此回顾冷冻切片技术在肌肉中的应用，根据目前已获得的以及预期可能获得的保存方面的改进来评估该技术，并讨论该技术在肌肉超微结构和功能研究中的许多潜在优势和用途。得出的结论是，该技术应大力发展，因为它有望在未来的肌肉研究中发挥非常重要的作用。
