Gu Z W, Whong W Z, Wallace W E, Ong T M
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888.
Mutat Res. 1992 Jun 1;279(3):217-22.
The genotoxicity of benzo[a]pyrene, cyclophosphamide, 2-aminoanthracene, 2-nitrofluorene, nitrosated coal-dust extracts, and cigarette-smoke condensate were tested with the micronucleus assay using an established mammalian cell line. The results showed that all chemicals and complex mixtures studied induced micronuclei in BALB/c-3T3 cells. These results indicate that BALB/c-3T3 cells are capable of activating certain promutagens and procarcinogens. It seems, therefore, that in addition to cell transformation, the micronucleus assay in BALB/c-3T3 cells without an exogenous activation system may be useful for in vitro studies to detect genotoxic chemicals and complex mixtures.
使用已建立的哺乳动物细胞系，通过微核试验检测了苯并[a]芘、环磷酰胺、2-氨基蒽、2-硝基芴、亚硝化煤尘提取物和香烟烟雾冷凝物的遗传毒性。结果表明，所研究的所有化学物质和复杂混合物均在BALB/c-3T3细胞中诱导产生微核。这些结果表明，BALB/c-3T3细胞能够激活某些前诱变剂和前致癌物。因此，似乎除了细胞转化外，在没有外源性激活系统的情况下，BALB/c-3T3细胞中的微核试验可能有助于体外研究检测遗传毒性化学物质和复杂混合物。
