Evaluation of frequency of micronuclei in exfoliated cells from bladder of mice treated with benzo(a) pyrene, 2-acetylaminofluorene and cyclophosphamide.

The micronucleus test (MNT) was applied in the epithelial cells from the bladder of mice treated with benzo(a)pyrene (BaP), 2-acetylaminofluorene (2-AAF) and cyclophosphamide (CP). Micronuclei (MN) were scored at different time on Feulgen and fast green stained smear preparations of exfoliated cells. These cells were obtained by scraping the internal surface of the bladder. The tested compounds gave the maximal response at 7-10 days after treatment. The number of MN in epithelial cells was dose dependent. These results suggest that exfoliated bladder cells from mice can be used as indicators for genotoxic damage in proliferating cell populations.