柴油颗粒中诱变成分的溶剂萃取效率。

Solvent extraction efficiencies of mutagenic components from diesel particles.

作者信息

Montreuil C N, Ball J C, Gorse R A, Young W C

机构信息

Ford Motor Company, Scientific Research Laboratory, Dearborn, MS 48121-2053.

出版信息

Mutat Res. 1992 Jun;282(2):89-92. doi: 10.1016/0165-7992(92)90079-w.

DOI:10.1016/0165-7992(92)90079-w

PMID:1377356

Abstract

In order to determine the efficiency of organic solvent extractions of fresh diesel-engine exhaust particles a series of Soxhlet extractions were set up using single and sequential extractions of fresh diesel particles with solvents of increasing polarities. Single extractions were carried out with methylene chloride, methanol, acetone and acetonitrile. A single filter was sequentially extracted with methylene chloride followed by methanol, acetone and acetonitrile. Methylene chloride extracted the most mutagenicity relative to the other three solvents. In addition, methylene chloride extracted 97% of the total extracted mutagenicity from a sequential series of extractions. Therefore, we conclude that of the solvents tested methylene chloride extracts the highest proportion of mutagens from fresh diesel exhaust and little mutagenicity is lost using methylene chloride as the only extraction solvent.

摘要

为了确定新鲜柴油发动机尾气颗粒的有机溶剂萃取效率，我们进行了一系列索氏萃取实验，采用单一萃取和连续萃取的方法，使用极性逐渐增加的溶剂对新鲜柴油颗粒进行萃取。单一萃取分别使用二氯甲烷、甲醇、丙酮和乙腈进行。对单个滤膜依次用二氯甲烷、甲醇、丙酮和乙腈进行萃取。相对于其他三种溶剂，二氯甲烷萃取的致突变性最强。此外，在连续萃取系列中，二氯甲烷萃取了总萃取致突变性的97%。因此，我们得出结论，在所测试的溶剂中，二氯甲烷从新鲜柴油尾气中萃取出的诱变剂比例最高，并且以二氯甲烷作为唯一萃取溶剂时几乎不会损失致突变性。