光果甘草提取物作为大肠杆菌K12+中拦截作用的效应物。

Glycyrrhiza glabra extract as an effector of interception in Escherichia coli K12+.

作者信息

Kuo S, Shankel D M, Telikepalli H, Mitscher L A

机构信息

Department of Microbiology, University of Kansas, Lawrence 66045.

出版信息

Mutat Res. 1992 Jun;282(2):93-8. doi: 10.1016/0165-7992(92)90080-2.

DOI:10.1016/0165-7992(92)90080-2

PMID:1377357

Abstract

Glycyrrhiza glabra polar lipid extract contains a number of flavonoids and related chemical compounds. Studies on the effectiveness of Glycyrrhiza glabra polar lipid extract in intercepting reactive molecules generated from the illumination of the photosensitizers rose bengal and phenosafranin indicate that it is effective in preventing cytotoxicity against E. coli K12+ in a dose-related fashion using illuminated rose bengal. Since only a modest scavenging of singlet oxygen generated from phenosafranin is observed, the effects of the extracts are less related to singlet oxygen-mediated oxidation of substrate (type II reactions) than non-singlet oxygen-mediated oxidation of substrate (type I reactions). Elevated levels of glutathione observed in exponentially growing cells of E. coli K12 were also observed.

摘要

光果甘草极性脂质提取物含有多种黄酮类化合物及相关化学物质。关于光果甘草极性脂质提取物对由孟加拉玫瑰红和番红精等光敏剂光照产生的反应性分子的拦截效果研究表明，在使用光照的孟加拉玫瑰红时，它能以剂量相关的方式有效预防对大肠杆菌K12 +的细胞毒性。由于仅观察到对番红精产生的单线态氧的适度清除，提取物的作用与单线态氧介导的底物氧化（II型反应）的相关性低于非单线态氧介导的底物氧化（I型反应）。在大肠杆菌K12的指数生长细胞中也观察到谷胱甘肽水平升高。