Department of Microbiology, Abdul Wali Khan University Mardan, Mardan, Pakistan.
Department of Microbiology, Kohat University of Science & Technology, Kohat, Pakistan.
BMC Complement Altern Med. 2018 May 2;18(1):140. doi: 10.1186/s12906-018-2207-8.
Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant's therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil.
The present investigation analyzed the High-Pressure Liquid Chromatography (HPLC) fractions of Glycyrrhiza glabra (Aqueous, Chloroform, Ethanol and Hexane) against multidrug resistant human bacterial pathogens (Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa). All the fractions showed antibacterial activity, were subjected to LC MS/MS analysis for identification of bioactive compounds.
Among total HPLC fractions of G. glabra (n = 20), three HPLC fractions showed potential activity against multidrug resistant (MDR) bacterial isolates. Fraction 1 (F1) of aqueous extracts, showed activity against A. baumannii (15 ± 0.5 mm). F4 from hexane extract of G. glabra showed activity against S. aureus (10 ± 0.2 mm). However, F2 from ethanol extract exhibited activity against S. aureus (10 ± 0.3 mm). These active fractions were further processed by LC MS/MS analysis for the identification of compounds. Ellagic acid was identified in the F1 of aqueous extract while 6-aldehydo-isoophiopogonone was present in F4 of hexane extract. Similarly, Liquirtigenin was identified in F2 of ethanol.
Glycyrrhiza glabra extracts HPLC fractions showed anti-MDR activity. Three bioactive compounds were identified in the study. 6-aldehydo-isoophiopogonone and Liquirtigenin were for the first time reported in G. glabra. Further characterization of the identified compounds will be helpful for possible therapeutic uses against infectious diseases caused by multidrug resistant bacteria.
药用植物已被发现作为全球传统草药。大多数植物的治疗特性是由于存在次生代谢产物，如生物碱、糖苷、单宁和挥发油。
本研究分析了甘草（水、氯仿、乙醇和正己烷）的高效液相色谱（HPLC）馏分对多药耐药的人类细菌病原体（大肠杆菌、鲍曼不动杆菌、金黄色葡萄球菌和铜绿假单胞菌）的作用。所有馏分均表现出抗菌活性，并用 LC-MS/MS 分析鉴定生物活性化合物。
在甘草（n=20）的总 HPLC 馏分中，有 3 个 HPLC 馏分显示出对多药耐药（MDR）细菌分离株的潜在活性。水提取物的 F1 显示对鲍曼不动杆菌的活性（15±0.5mm）。正己烷提取物的 F4 对金黄色葡萄球菌表现出活性（10±0.2mm）。然而，F2 对金黄色葡萄球菌表现出活性（10±0.3mm）。这些活性馏分进一步通过 LC-MS/MS 分析进行化合物鉴定。在水提取物的 F1 中鉴定出鞣花酸，而 F4 中存在 6-醛基异麦冬酮。同样，在乙醇提取物的 F2 中鉴定出甘草素。
甘草提取物的 HPLC 馏分显示出抗 MDR 活性。本研究鉴定出 3 种生物活性化合物。6-醛基异麦冬酮和甘草素是首次在甘草中报道的。对鉴定出的化合物进行进一步的表征将有助于对抗由多药耐药细菌引起的传染病的可能治疗用途。
