Ko R C, Yeung M H
Department of Zoology, University of Hong Kong.
Vet Parasitol. 1992 Apr;42(1-2):101-10. doi: 10.1016/0304-4017(92)90106-j.
A modified enhanced chemiluminescent enzyme assay (ECIA) was developed for mass screening of pigs for trichinellosis antibodies in abattoirs. Using Bionectics beads as solid support, the assay time could be reduced to 45 min. Optimal conditions for washing, blocking, incubation, concentration of serum, antigens and conjugates as well as timing of film exposure were determined. The sensitivity and specificity of the assay were found to be comparable to those of the triple antibody-IgG ELISA. The assay was tested in an abattoir and its efficacy was found to be satisfactory. However, the major disadvantage of the assay is the high cost of magnetic beads.
开发了一种改良的增强化学发光酶分析法（ECIA），用于在屠宰场对猪进行旋毛虫病抗体的大规模筛查。使用Bionectics磁珠作为固相载体，分析时间可缩短至45分钟。确定了洗涤、封闭、孵育、血清、抗原和结合物浓度以及胶片曝光时间的最佳条件。发现该分析法的敏感性和特异性与三联抗体-IgG ELISA相当。该分析法在一家屠宰场进行了测试，发现其效果令人满意。然而，该分析法的主要缺点是磁珠成本高。
