Zhang Gai-Ping, Guo Junq-Qing, Wang Xuan-Nian, Yang Jun-Xing, Yang Yan-Yan, Li Qing-Mei, Li Xue-Wu, Deng Rui-Guang, Xiao Zhi-Jun, Yang Ji-Fei, Xing Guang-Xu, Zhao Dong
Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Vet Parasitol. 2006 Apr 30;137(3-4):286-93. doi: 10.1016/j.vetpar.2006.01.026. Epub 2006 Feb 17.
In the present study, an immunochromatographic strip was developed for the serological detection of trichinellosis in swine. In the strip, the excretory-secretory (ES) antigens of Trichinella labelled with colloidal gold was used as the detector, and the staphylococcal protein A (SPA) and goat anti-ES antibody were blotted on the nitrocellulose membrane for the test and control lines, respectively. The evaluation of the strip was performed by comparing 60 clinical positive blood samples detected by the artificial digestion method with 46 serum samples from pigs infected with parasites other than Trichinella and 30 serum samples of parasite-free healthy pigs. The strip was shown to be of high specificity and sensitivity that were closely correlated with those of ELISA. Furthermore, the dipstick assay based on the strip is rapid (10 min) and easy to perform with no requirement of special skill, reagent or equipment. This suggests the immunochromatographic strip is an acceptable alternative to be used in clinical laboratories lacking specialized equipment as well as for field diagnosis.
在本研究中，开发了一种免疫层析试纸条用于猪旋毛虫病的血清学检测。在试纸条中，用胶体金标记的旋毛虫排泄分泌（ES）抗原作为检测物，葡萄球菌蛋白A（SPA）和山羊抗ES抗体分别印迹在硝酸纤维素膜上作为检测线和对照线。通过将人工消化法检测的60份临床阳性血样与46份感染非旋毛虫寄生虫的猪血清样本以及30份无寄生虫健康猪的血清样本进行比较，对试纸条进行了评估。结果表明，该试纸条具有高特异性和高灵敏度，与酶联免疫吸附测定（ELISA）密切相关。此外，基于该试纸条的快速检测法耗时短（10分钟），操作简便，无需特殊技能、试剂或设备。这表明免疫层析试纸条是临床实验室缺乏专业设备时以及现场诊断的一种可接受的替代方法。
