Haines J W
National Radiological Protection Board, Didcot, Oxon, United Kingdom.
Biotech Histochem. 1992 Jan;67(1):45-9. doi: 10.3109/10520299209110005.
A method has been developed by which large samples of mineralized bone, containing an alpha-emitter, can be embedded in Spurr's resin in a fraction of the time required by conventional methods. Bone samples were freeze-dried or fixed and dried prior to impregnation with Spurr's resin under vacuum. Sections were cut for the preparation of either alpha-track or fission-track autoradiographs using the solid state detector CR-39. This method is applicable to samples containing a mobile form of a radionuclide that may be translocated during the processes of fixation and dehydration of the specimen.
已开发出一种方法，通过该方法，含有α发射体的大量矿化骨样本能够在传统方法所需时间的一小部分内被嵌入Spurr树脂中。骨样本在真空下用Spurr树脂浸渍之前进行冷冻干燥或固定并干燥。使用固态探测器CR-39切割切片以制备α径迹或裂变径迹放射自显影片。该方法适用于含有可在标本固定和脱水过程中发生移位的放射性核素移动形式的样本。
