TELFER W H
J Biophys Biochem Cytol. 1961 Apr;9(4):747-59. doi: 10.1083/jcb.9.4.747.
The oocytes of saturniid moths take up proteins selectively from the blood. The distribution of blood proteins in the ovary during protein uptake was investigated by staining 2 micro sections of freeze-dried ovaries with fluorescein-labeled antibodies. The results indicate that blood proteins occur primarily in the intercellular spaces of the follicle cell layer, in association with a brush border at the surface of the oocyte, and within the oocyte in the yolk spheres. That proteins derived from the blood are associated with the yolk spheres was confirmed by isolating these bodies and showing that lysis, which can be induced by any of a number of mechanical means, causes them to release immunologically defined proteins known to be derived from the blood. That the level of blood proteins in the cytoplasm is low relatively to that in the yolk spheres was confirmed by the observation that the yellow pigments associated with several blood proteins, although conspicuous in the yolk spheres, are not visible in the translucent layer of centrifuged oocytes. From these and previous physiological observations, it is proposed that blood proteins reach the surface of the oocyte by an intercellular route, that they combine with some component of the brush border, and that they are transformed into yolk spheres by a process akin to pinocytosis.
天蚕蛾的卵母细胞从血液中选择性摄取蛋白质。通过用荧光素标记的抗体对冻干卵巢的2微米切片进行染色，研究了蛋白质摄取过程中血液蛋白质在卵巢中的分布。结果表明，血液蛋白质主要存在于卵泡细胞层的细胞间隙中，与卵母细胞表面的刷状缘相关，并且存在于卵黄球中的卵母细胞内。通过分离这些球体并表明多种机械手段中的任何一种都能诱导其裂解，从而导致它们释放已知源自血液的免疫定义蛋白质，证实了源自血液的蛋白质与卵黄球相关。通过观察发现，与几种血液蛋白质相关的黄色色素虽然在卵黄球中很明显，但在离心卵母细胞的半透明层中不可见，这证实了细胞质中血液蛋白质的水平相对于卵黄球中的水平较低。根据这些以及先前的生理学观察结果，有人提出血液蛋白质通过细胞间途径到达卵母细胞表面，它们与刷状缘的某些成分结合，并且它们通过类似于胞饮作用的过程转化为卵黄球。
