Selman K, Wallace R A
Tissue Cell. 1982;14(3):555-71. doi: 10.1016/0040-8166(82)90047-7.
The structure of the vitellogenic follicles of the sheepshead minnow, Cyprinodon variegatus, is described. Follicles enlarge primarily by protein yolk accumulation (vitellogenesis) and subsequently increase in size by hydration. This study uses the electron-dense tracer, horseradish peroxidase, and a larger heterologous protein, Xenopus laevis [3H]vitellogenin, to follow the fate of exogenous proteins from the maternal circulation to yolk spheres of the growing oocyte. Materials appear to leave the perifollicular capillaries via an interendothelial route, traverse the theca and the patent intercellular channels of the follicular epithelium and the pore canals of the vitelline envelope. At the oocyte surface they are incorporated via micropinocytosis and translocated to growing yolk spheres in the peripheral ooplasm. In contrast to other studies on oocyte growth in teleosts which suggest that yolk is an autosynthetic product, this study substantiates the importance of heterosynthetic processes during oocyte growth in C. variegatus.
描述了羊头鲦鱼（Cyprinodon variegatus）卵黄生成卵泡的结构。卵泡主要通过蛋白质卵黄积累（卵黄生成）而增大，随后通过水合作用进一步增大尺寸。本研究使用电子致密示踪剂辣根过氧化物酶和一种较大的异源蛋白——非洲爪蟾[3H]卵黄蛋白原，来追踪外源蛋白从母体循环到生长中卵母细胞的卵黄球的命运。物质似乎通过内皮间途径离开卵泡周围毛细血管，穿过卵泡膜以及卵泡上皮细胞间的开放通道和卵黄膜的孔道。在卵母细胞表面，它们通过微胞饮作用被摄取，并转运到外周卵质中正在生长的卵黄球。与其他关于硬骨鱼卵母细胞生长的研究表明卵黄是自体合成产物不同，本研究证实了异源合成过程在羊头鲦鱼卵母细胞生长中的重要性。
