Larkin M, Ahern T J, Stoll M S, Shaffer M, Sako D, O'Brien J, Yuen C T, Lawson A M, Childs R A, Barone K M
Glycoconjugates Section, Medical Research Council Clinical Research Centre, Harrow, Middlesex, United Kingdom.
J Biol Chem. 1992 Jul 5;267(19):13661-8.
Carbohydrate recognition by the human endothelial-leukocyte adhesion molecule, E-selectin, has been investigated by binding studies using 3H-labeled Chinese hamster ovary cells expressing different levels of the transfected full-length adhesion molecule and a series of structurally defined oligosaccharides linked to the lipid phosphatidylethanolamine dipalmitoate (neoglycolipids) and synthetic glycolipids chromatographed on silica gel plates or immobilized on plastic wells. Evidence is presented for density-dependent binding of the membrane-associated E-selectin not only to 3'-sialyl-lacto-N-fucopentaose II (3'-S-LNFP-II) and 3'-sialyl-lacto-N-fucopentaose III (3'-S-LNFP-III) which express the sialyl Le(a) and sialyl Le(x) antigens, respectively, but also to the nonsialylated analogue LNFP-II; there is a threshold density of E-selectin required for binding to these sialylated sequences, and binding to the nonsialylated analogue is a property only of cells with the highest density of E-selectin expression. The presence of fucose linked to subterminal rather than to an internal N-acetylglucosamine is shown to be a requirement for E-selectin binding, and although the presence of sialic acid 3-linked to the terminal galactose of the LNFP-II or LNFP-III sequences substantially enhances E-selectin binding, the presence of 6-linked sialic acid abolishes binding. E-selectin binding is unaffected in the presence of the blood group H fucose (alpha 1-2 linked to galactose to form the Le(b) antigen). However, the binding is abolished when in addition alpha 1-3-linked N-acetylgalactosamine to the galactose (blood group A antigen) is present. These results indicate that some E-selectin-mediated adhesive events may be influenced by blood group status.
通过结合研究，利用表达不同水平转染全长黏附分子的3H标记中国仓鼠卵巢细胞以及一系列与脂质二棕榈酰磷脂酰乙醇胺相连的结构明确的寡糖（新糖脂）和在硅胶板上进行色谱分析或固定在塑料孔中的合成糖脂，对人内皮细胞-白细胞黏附分子E-选择素的碳水化合物识别进行了研究。有证据表明，膜相关的E-选择素不仅与分别表达唾液酸化Le(a)和唾液酸化Le(x)抗原的3'-唾液酸乳糖-N-岩藻五糖II（3'-S-LNFP-II）和3'-唾液酸乳糖-N-岩藻五糖III（3'-S-LNFP-III）存在密度依赖性结合，还与非唾液酸化类似物LNFP-II存在密度依赖性结合；与这些唾液酸化序列结合需要一定的E-选择素阈值密度，并且与非唾液酸化类似物的结合仅是E-选择素表达密度最高的细胞的特性。研究表明，与亚末端而非内部N-乙酰葡糖胺相连的岩藻糖的存在是E-选择素结合的必要条件，尽管与LNFP-II或LNFP-III序列末端半乳糖相连的3-唾液酸的存在显著增强了E-选择素结合，但6-唾液酸的存在会消除结合。在血型H岩藻糖（α1-2连接到半乳糖形成Le(b)抗原）存在的情况下，E-选择素结合不受影响。然而，当除了α1-3连接的N-乙酰半乳糖胺连接到半乳糖（血型A抗原）也存在时，结合会被消除。这些结果表明，一些E-选择素介导的黏附事件可能受血型状态的影响。
