Green P J, Tamatani T, Watanabe T, Miyasaka M, Hasegawa A, Kiso M, Yuen C T, Stoll M S, Feizi T
Glycoconjugates Section, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
Biochem Biophys Res Commun. 1992 Oct 15;188(1):244-51. doi: 10.1016/0006-291x(92)92376-9.
The binding of the leucocyte adhesion molecule L-selectin has been investigated toward several structurally defined lipid-linked oligosaccharides immobilized on silica gel chromatograms or plastic wells. In both assay systems the 3'-sulphated Le(a)/Le(x) type tetrasaccharides [formula: see text] were more strongly bound than 3'-sialyl analogues. A considerable binding was observed to the 3'-sulphated oligosaccharide backbone in the absence of fucose but not to a 3'-sialyl analogue or fuco-oligosaccharide analogues lacking sulphate or sialic acid. Affinity for other sulphated saccharides: 3'-sulphoglucuronyl neolactotetraosyl ceramide and glycolipids with sulphate 3'-linked to terminal or sub-terminal galactose or N-acetylgalactosamine was detected in the chromatogram assay only. These studies, together with earlier reports that L-selectin binding to endothelium is inhibited by sulphatide, highlight the relative importance of sulphate in the adhesive specificity of this protein.
人们已经研究了白细胞粘附分子L-选择素与固定在硅胶色谱图或塑料孔上的几种结构明确的脂连接寡糖的结合情况。在这两种检测系统中，3'-硫酸化的Le(a)/Le(x)型四糖[化学式：见原文]比3'-唾液酸类似物结合得更强。在没有岩藻糖的情况下，观察到3'-硫酸化寡糖主链有相当程度的结合，但对于3'-唾液酸类似物或缺乏硫酸盐或唾液酸的岩藻糖寡糖类似物则没有观察到结合。仅在色谱图检测中检测到对其他硫酸化糖类的亲和力：3'-硫酸化葡糖醛酸新乳糖四糖神经酰胺以及硫酸盐与末端或亚末端半乳糖或N-乙酰半乳糖胺3'-相连的糖脂。这些研究，连同早期关于硫脂抑制L-选择素与内皮细胞结合的报道，突出了硫酸盐在该蛋白粘附特异性中的相对重要性。
