N,N'-二甲基脲和盐酸甲胺在艾姆斯沙门氏菌/微粒体试验中的致突变性：无致突变反应。

Mutagenicity of N,N'-dimethylurea and methylamine hydrochloride in the Ames Salmonella/microsome test: absence of mutagenic response.

作者信息

Meshram G P, Malini R P, Rao K M

机构信息

Division of Microbiology, Defence Research and Development Establishment, Gwalior, India.

出版信息

Mutat Res. 1992 Jun 16;279(4):275-80. doi: 10.1016/0165-1218(92)90244-t.

DOI:10.1016/0165-1218(92)90244-t

PMID:1377783

Abstract

Methyl isocyanate (MIC) in aqueous solution forms methylamine (MA) and N,N'-dimethylurea (DMU). MA in buffered system further converts into its salt form, methylamine hydrochloride (MAH). Therefore, MAH and DMU were evaluated for their mutagenic activity in the in vitro Ames Salmonella/microsome mutagenicity test. The liquid preincubation protocol was followed, using tester strains TA98, TA100 and TA104 of Salmonella typhimurium, in the presence of 0, 5, 15 and 30% Aroclor 1254-induced rat liver S9 mixture. DMU and MAH did not induce a mutagenic response in any of the tester strains, both in the presence and in the absence of S9 mixture. The results therefore confirm that MIC in its native form or as its unknown metabolites is responsible for the mutagenic activity reported earlier by us in the his tester strains TA100 and TA104 of Salmonella typhimurium (Mutation Res., 204 (1988) 123-129) and not due to its hydrolysis products, MA or DMU.

摘要

异氰酸甲酯（MIC）在水溶液中会形成甲胺（MA）和N，N'-二甲基脲（DMU）。在缓冲体系中，甲胺会进一步转化为其盐形式，即盐酸甲胺（MAH）。因此，在体外艾姆斯沙门氏菌/微粒体诱变试验中对盐酸甲胺和N，N'-二甲基脲的诱变活性进行了评估。采用液体预孵育方案，使用鼠伤寒沙门氏菌的测试菌株TA98、TA100和TA104，同时加入0%、5%、15%和30%的艾氏剂1254诱导的大鼠肝脏S9混合物。无论有无S9混合物，N，N'-二甲基脲和盐酸甲胺在任何测试菌株中均未诱导出诱变反应。因此，结果证实，我们之前报道的在鼠伤寒沙门氏菌的his测试菌株TA100和TA104中观察到的诱变活性（《突变研究》，204（1988）123 - 129）是由异氰酸甲酯的天然形式或其未知代谢产物引起的，而非其水解产物甲胺或N，N'-二甲基脲所致。