香兰素对黑腹果蝇生殖细胞系中自发产生或由丝裂霉素C诱导产生的染色体畸变的抑制作用。

Suppressing effect of vanillin on chromosome aberrations that occur spontaneously or are induced by mitomycin C in the germ cell line of Drosophila melanogaster.

作者信息

de Andrade H H, Santos J H, Gimmler-Luz M C, Correa M J, Lehmann M, Reguly M L

机构信息

Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

出版信息

Mutat Res. 1992 Jun 16;279(4):281-7. doi: 10.1016/0165-1218(92)90245-u.

DOI:10.1016/0165-1218(92)90245-u

PMID:1377784

Abstract

In order to investigate the anticlastogenic effect of vanillin on ring-X loss, D. melanogaster females exposed to different vanillin concentrations were crossed with non-treated, MMC- or MMS-treated males. The results obtained with this in vivo investigation showed a significant inhibition of vanillin in the frequencies of spontaneous ring-X loss--59, 56, 38 and 36%--at the different concentrations used. In addition, vanillin treatment caused a significant suppression of MMC-induced ring-X loss. This decrease was observed only in the first 3 days after the interruption of vanillin treatment and at the concentrations of 0.5 and 1% of this flavoring agent. In contrast, vanillin did not show any effect on chromosome loss provoked by MMS. Therefore, the ring-X loss-decreasing effect of vanillin seemed to depend on the quality of DNA lesions and consequently on a specific enzymatic repair process present in the oocytes of D. melanogaster.

摘要

为了研究香草醛对X染色体环缺失的抗致断裂作用，将暴露于不同浓度香草醛的黑腹果蝇雌性与未处理、经丝裂霉素C（MMC）或甲基磺酸甲酯（MMS）处理的雄性果蝇进行杂交。这项体内研究获得的结果表明，在所使用的不同浓度下，香草醛对自发X染色体环缺失频率有显著抑制作用，抑制率分别为59%、56%、38%和36%。此外，香草醛处理可显著抑制MMC诱导的X染色体环缺失。仅在中断香草醛处理后的前3天以及该调味剂浓度为0.5%和1%时观察到这种减少。相比之下，香草醛对MMS引发的染色体缺失没有任何影响。因此，香草醛的X染色体环缺失减少作用似乎取决于DNA损伤的性质，进而取决于黑腹果蝇卵母细胞中存在的特定酶促修复过程。