Andresen I, Becker W, Schlüter K, Burges J, Parthier B, Apel K
Botanisches Institut, Universität Kiel, Germany.
Plant Mol Biol. 1992 May;19(2):193-204. doi: 10.1007/BF00027341.
Jasmonic acid (JA) and its methyl ester (JA-Me) are able to introduce the accumulation of several specific polypeptides in cut leaf segments of barley. Two of the most prominent JA-induced proteins of M(r) 15,000 and 23,000 have been characterized by isolating and sequencing complete cDNA sequences. While the sequence of the M(r) 23,000 polypeptide shows no similarity to published sequences, the sequence of the M(r) 15,000 polypeptide corresponds to the higher-molecular-weight precursor of a leaf thionin previously characterized. Transcripts for the M(r) 23,000 and M(r) 15,000 polypeptides accumulate in leaf segments shortly after the beginning of JA treatment. JA and JA-Me induce the appearance of the two proteins not only in leaf segments but also in intact barley seedlings. However, in seedlings the accumulation of JA-induced proteins occurs much more slowly and requires high concentrations of volatile JA-Me. Thus, in barley it seems unlikely that volatile JA-Me is involved in the interaction between different members of this species, as has been proposed recently for tomato seedlings.
茉莉酸（JA）及其甲酯（JA-Me）能够促使大麦离体叶片切段中积累多种特定的多肽。通过分离和测定完整的cDNA序列，已对两种最显著的由JA诱导产生的蛋白质（分子量分别为15,000和23,000）进行了表征。虽然分子量为23,000的多肽序列与已发表的序列没有相似性，但分子量为15,000的多肽序列与先前表征的一种叶片硫堇的高分子量前体相对应。在JA处理开始后不久，叶片切段中就会积累分子量为23,000和15,000的多肽的转录本。JA和JA-Me不仅能在叶片切段中诱导这两种蛋白质的出现，在完整的大麦幼苗中也能诱导其出现。然而，在幼苗中，JA诱导蛋白质的积累要慢得多，并且需要高浓度的挥发性JA-Me。因此，在大麦中，挥发性JA-Me似乎不太可能参与该物种不同成员之间的相互作用，而最近有人提出这种相互作用存在于番茄幼苗中。
