Department of Agronomy, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0915.
Plant Physiol. 1991 May;96(1):130-6. doi: 10.1104/pp.96.1.130.
Vegetative storage protein (VSP) and VSP mRNA levels in soybean (Glycine max) leaves correlated with the amount of NH(4)NO(3) provided to nonnodulated plants. The mRNA level declined as leaves matured, but high levels of N delayed the decline. This is consistent with the proposed role for VSP in the temporary storage of N. Wounding, petiole girdling, and treatment with methyljasmonate (MeJA) increased VSP mRNA in leaves 24 hours after treatment. The magnitude of the response depended on leaf age and N availability. N deficiency essentially eliminated the response to wounding and petiole girdling. MeJA was almost as effective in N-deficient plants as in those receiving abundant N. Inhibitors of lipoxygenase, the first enzyme in the jasmonic acid biosynthetic pathway, blocked induction by wounding and petiole girdling but not by MeJA. This supports a role for endogenous leaf jasmonic acid (or MeJA) in the regulation of VSP gene expression.
植物营养体贮藏蛋白（VSP）及其 mRNA 水平与非根瘤大豆（Glycine max）叶片中 NH4NO3的含量相关。随着叶片的成熟，mRNA 水平下降，但高浓度的 N 会延缓下降。这与 VSP 在 N 临时储存中的作用相一致。伤流、叶柄环割和茉莉酸甲酯（MeJA）处理均可增加处理后 24 小时叶片中的 VSP mRNA。响应的幅度取决于叶片的年龄和 N 的可用性。N 缺乏基本上消除了对伤流和叶柄环割的响应。MeJA 在 N 缺乏的植物中的有效性几乎与在那些接受大量 N 的植物中相同。脂氧合酶抑制剂，即茉莉酸生物合成途径中的第一个酶，阻断了伤流和叶柄环割的诱导，但不阻断 MeJA 的诱导。这支持了内源性叶片茉莉酸（或 MeJA）在 VSP 基因表达调控中的作用。
