Turpeenniemi-Hujanen T, Rönnberg L, Kauppila A, Puistola U
Department of Radiotherapy and Oncology, University of Oulu, Finland.
Fertil Steril. 1992 Jul;58(1):105-13. doi: 10.1016/s0015-0282(16)55145-0.
To explore the possible similarities between the biochemical processes of embryo implantation and malignant invasion.
The expression of a basement membrane (BM) glycoprotein laminin, a matrix binding cell surface receptor protein beta 1-integrin, and a BM collagen degrading metalloproteinase type IV collagenase, was studied in cultured human in vitro fertilized embryos.
Eight healthy women suffering from tubal infertility were participating in the IVF program in the Department of Obstetrics and Gynecology in University Hospital of Oulu. Twenty oocytes and 110 pre-embryos that were not transferred for the fertilizations were used in this study.
Fibronectin, laminin, beta 1-integrin, and type IV collagenase immunoreactive proteins were studied in embryos by immunoperoxidase staining, and type IV collagen degrading activity was measured from the culture media of the embryos.
Laminin and beta 1-integrin were expressed in the early human embryos before the time of implantation. Type IV collagen degrading activity and the 72 kd-type IV collagenase immunoreactive protein were expressed at the time of implantation. Laminin supported the expression of type IV collagenase.
The expression of laminin, beta 1-integrin, and type IV collagenase in vitro are temporally in good correlation with the time of the implantation in vivo. Laminin and beta 1-integrin can relate to the attachment of the embryos to the uterine BM and type IV collagenase to the degradation of the BM collagen during the implantation. Laminin can augment the process locally.
探讨胚胎着床与恶性侵袭生化过程之间可能存在的相似性。
研究培养的人类体外受精胚胎中基底膜（BM）糖蛋白层粘连蛋白、基质结合细胞表面受体蛋白β1整合素以及BM胶原降解金属蛋白酶IV型胶原酶的表达。
8名患有输卵管性不孕的健康女性参与了奥卢大学医院妇产科的体外受精项目。本研究使用了20个未用于受精移植的卵母细胞和110个胚胎前体。
通过免疫过氧化物酶染色研究胚胎中的纤连蛋白、层粘连蛋白、β1整合素和IV型胶原酶免疫反应蛋白，并从胚胎培养基中测量IV型胶原降解活性。
层粘连蛋白和β1整合素在人类早期胚胎着床前就已表达。IV型胶原降解活性和72kd-IV型胶原酶免疫反应蛋白在着床时表达。层粘连蛋白支持IV型胶原酶的表达。
体外层粘连蛋白、β1整合素和IV型胶原酶的表达与体内着床时间在时间上具有良好的相关性。层粘连蛋白和β1整合素可能与胚胎附着于子宫BM有关，而IV型胶原酶与着床期间BM胶原的降解有关。层粘连蛋白可在局部增强这一过程。
