大鼠脑碱性成纤维细胞生长因子（bFGF）mRNA的PCR检测，该mRNA含有独特的3'非翻译区。
PCR detection of the rat brain basic fibroblast growth factor (bFGF) mRNA containing a unique 3' untranslated region.
作者信息
el-Husseini A E, Paterson J A, Myal Y, Shiu R P
机构信息
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
出版信息
Biochim Biophys Acta. 1992 Jul 15;1131(3):314-6. doi: 10.1016/0167-4781(92)90031-t.
We utilized the reverse transcription-polymerase chain reaction (RT-PCR) to detect the presence of basic fibroblast growth factor (bFGF) messenger RNA in rat brain, ovary and kidney. The nucleotide sequence of the RT-PCR product revealed a novel 3' untranslated (UT) sequence in the rat basic FGF mRNA. In this sequence, as in the 3' UT regions of many growth regulatory genes, there is a high degree of conservation of A+T rich motifs which have been shown to play a major role in mRNA stability.
我们利用逆转录-聚合酶链反应（RT-PCR）来检测大鼠脑、卵巢和肾脏中碱性成纤维细胞生长因子（bFGF）信使核糖核酸（mRNA）的存在。RT-PCR产物的核苷酸序列揭示了大鼠碱性FGF mRNA中一个新的3'非翻译（UT）序列。在这个序列中，如同许多生长调节基因的3'UT区域一样，富含A+T的基序具有高度保守性，这些基序已被证明在mRNA稳定性中起主要作用。
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