Paterson J A, Shiu R P
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Mol Cell Endocrinol. 1994 Sep;104(2):191-200. doi: 10.1016/0303-7207(94)90122-8.
Regional and temporal patterns of the expression of basic fibroblast growth factor (bFGF), and two of its high affinity receptors (FGFR1 and FGFR2), were examined in the male rat brain during early postnatal development; the reverse transcription-polymerase chain reaction (RT-PCR) was used to obtain mRNA measurements which were expressed relative to mRNA for GAPDH as a constant. In the rat cerebrum, the mRNAs for bFGF and for FGFR2 were relatively low in amount within the first postnatal week, but by 28 days, they were as high as in the 1-year-old rat cerebrum. In contrast, the expression of FGFR1 was biphasic: mRNA levels were higher at postnatal days 1 and 28 than at day 21. Quantitation of mRNA from microdissected regions of 28-day-old rat brain revealed that the expression of bFGF and of FGFR2 showed a marked variation between regions but the expression of FGFR1 appeared less variable between the regions that were analyzed. For all three genes the hippocampus appeared to have high relative amounts of mRNA. The temporal patterns of expression of bFGF, FGFR1 and FGFR2 also differed with brain region during early postnatal development. In the occipital cortex and inferior colliculus, the mRNAs for bFGF and FGFR2 both increased in amount during the first month, unlike that for FGFR1. However, in the cerebellum, the highest expression of bFGF and FGFR1 mRNAs occurred at postnatal day 1; FGFR2 expression apparently showed less change with age. The temporal changes in bFGF, FGFR1 and FGFR2 expression in different brain regions during early postnatal development suggest that receptor regulation may permit different physiological effects of bFGF according to brain region and developmental age.
在出生后早期发育阶段，对雄性大鼠脑内碱性成纤维细胞生长因子（bFGF）及其两种高亲和力受体（FGFR1和FGFR2）的表达区域和时间模式进行了检测；采用逆转录聚合酶链反应（RT-PCR）获得mRNA测量值，并将其相对于甘油醛-3-磷酸脱氢酶（GAPDH）的mRNA作为常量进行表达。在大鼠大脑中，出生后第一周内bFGF和FGFR2的mRNA含量相对较低，但到28天时，它们的含量与1岁大鼠大脑中的含量一样高。相比之下，FGFR1的表达呈双相性：出生后第1天和第28天的mRNA水平高于第21天。对28日龄大鼠脑显微切割区域的mRNA定量分析表明，bFGF和FGFR2的表达在不同区域之间存在显著差异，但FGFR1的表达在所分析的区域之间变化较小。对于所有这三个基因，海马体似乎具有相对较高的mRNA含量。在出生后早期发育阶段，bFGF、FGFRl和FGFR2的表达时间模式也因脑区而异。在枕叶皮质和下丘脑中，bFGF和FGFR2的mRNA含量在第一个月内均增加，这与FGFR1不同。然而，在小脑中，bFGF和FGFR1 mRNA的最高表达出现在出生后第1天；FGFR2的表达显然随年龄变化较小。出生后早期发育阶段不同脑区bFGF、FGFR1和FGFR2表达的时间变化表明，受体调节可能使bFGF根据脑区和发育年龄产生不同的生理效应。
