Gourdin M F, Reyes F, Lejonc J L, Mannoni P, Dreyfus B
Hamatol Bluttransfus. 1976;19:207-19. doi: 10.1007/978-3-642-87524-3_22.
Peroxidase conjugated antibodies were applied to cell suspensions in order to detect surface associated immunoglobulins. Cell suspensions were fixed prior to incubation with reagents, a procedure avoiding membrane alterations induced by antibodies to surface component. By immunoelectron microscopy an identification of B lymphocytes could be made with simultaneous observation of their surface architecture. Basic findings were that normal circulating human B lymphocytes had a villous surface. This relationship was not confirmed however by examinating samples from various B and T cell proliferations establishing that surface morphology is not sufficient to categorize cells in disease. Specimens from hairy cell leukemia were also examined. Despite salient surface characteristics as revealed by the present method, the categorization of cells remains unclear.
将过氧化物酶偶联抗体应用于细胞悬液，以检测表面相关免疫球蛋白。细胞悬液在与试剂孵育前进行固定，这一程序可避免因针对表面成分的抗体引起的膜改变。通过免疫电子显微镜，可以在同时观察其表面结构的情况下对B淋巴细胞进行鉴定。基本发现是，正常循环的人B淋巴细胞具有绒毛状表面。然而，通过检查来自各种B细胞和T细胞增殖的样本，并未证实这种关系，这表明表面形态不足以对疾病中的细胞进行分类。还检查了毛细胞白血病的标本。尽管本方法揭示了显著的表面特征，但细胞的分类仍不明确。
