Reyes F, Gourdin M F, Farcet J P, Dreyfus B
Ann Med Interne (Paris). 1976 Dec;127(12):865-72.
Surface associated immunoglobulins (s.Ig) have been detected on human lymphocytes, in normal individuals and in disease, by an immunoelectron microscopic method using peroxidase-labeled antibodies. Experiments have been carried out on fixed cell suspensions, in order to avoid membrane alterations induced by anti-immunoglobulin antibodies. Normal human blood B lymphocytes have a villous surface. However this relationship between microvilli and detectable s.Ig, as found in the normal state, is not confirmed by examinating various T and B cell proliferative states. Thus surface morphology alone is not sufficient for classifying cells in disease. The precise nature of mononuclear cells from hairy cell leukemia remains nuclear.
通过使用过氧化物酶标记抗体的免疫电子显微镜方法，已在正常个体和患病个体的人淋巴细胞上检测到表面相关免疫球蛋白（s.Ig）。实验是在固定的细胞悬液上进行的，以避免抗免疫球蛋白抗体引起的膜改变。正常人类血液B淋巴细胞具有绒毛状表面。然而，在检查各种T和B细胞增殖状态时，并未证实正常状态下发现的微绒毛与可检测到的s.Ig之间的这种关系。因此，仅表面形态不足以对疾病中的细胞进行分类。毛细胞白血病单核细胞的确切性质仍然未知。
