Zauli G, Vitale L, Brunelli M A, Bagnara G P
Institute of Histology and General Embryology, University of Bologna, Italy.
Exp Hematol. 1992 Aug;20(7):850-4.
The in vitro growth of early (megakaryocyte burst-forming units, BFU-meg) and late (megakaryocyte colony-forming units, CFU-meg) megakaryocyte (meg) progenitors has been evaluated in normal adult human peripheral blood (PB). All the experiments were carried out using CD34+ cells, which were assayed in a serum-free fibrinclot assay. PB BFU-meg were morphologically characterized as plurifocal aggregates containing greater than 50 cells/colony, distinct from unifocal CFU-meg, in a limiting dilution assay. At variance with PB CFU-meg, PB BFU-meg were unaffected by the complement-mediated cytotoxicity with anti-HLA-DR. The optimal source of colony-stimulating activity for PB BFU-meg growth was recombinant human interleukin 3 (rhIL-3; 100 U/ml), which supported a significantly higher number of BFU-meg in comparison with recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF; 200 U/ml, p = 0.043). Combinations of rhIL-3 (100 U/ml) plus rhGM-CSF (200 U/ml), rhIL-3 plus recombinant human interleukin 6 (rhIL-6; 100 U plus 100 U/ml) or rhIL-3 plus rhGM-CSF plus rhIL-6 (100 U plus 200 U/ml plus 100 U/ml) failed to further increase the number of PB BFU-meg with respect to rhIL-3 (100 U/ml) alone. Both PB BFU-meg and CFU-meg were markedly inhibited, in a dose-dependent fashion, by increasing doses of human purified transforming growth factor-beta 1 (TGF-beta 1) (from 0.001 to 10 ng/ml). Finally, the CFU-meg/BFU-meg ratio in PB (0.52) was significantly different from that of normal bone marrow (2.3), clearly indicating that adult human peripheral blood predominantly carries primitive megakaryocytic progenitors.
已对正常成人外周血（PB）中早期（巨核细胞爆式集落形成单位，BFU - meg）和晚期（巨核细胞集落形成单位，CFU - meg）巨核细胞（meg）祖细胞的体外生长情况进行了评估。所有实验均使用CD34⁺细胞，在无血清纤维蛋白凝块试验中进行检测。在有限稀释试验中，PB BFU - meg在形态上的特征为多灶性聚集物，每个集落包含超过50个细胞，这与单灶性CFU - meg不同。与PB CFU - meg不同，PB BFU - meg不受抗HLA - DR补体介导的细胞毒性影响。PB BFU - meg生长的集落刺激活性的最佳来源是重组人白细胞介素3（rhIL - 3；100 U/ml），与重组人粒细胞 - 巨噬细胞集落刺激因子（rhGM - CSF；200 U/ml，p = 0.043）相比，它支持的BFU - meg数量显著更多。rhIL - 3（100 U/ml）加rhGM - CSF（200 U/ml）、rhIL - 3加重组人白细胞介素6（rhIL - 6；100 U加100 U/ml）或rhIL - 3加rhGM - CSF加rhIL - 6（100 U加200 U/ml加100 U/ml）的组合相对于单独的rhIL - 3（100 U/ml）未能进一步增加PB BFU - meg的数量。随着人纯化转化生长因子 - β1（TGF - β1）剂量增加（从0.001至10 ng/ml），PB BFU - meg和CFU - meg均以剂量依赖性方式受到显著抑制。最后，PB中的CFU - meg/BFU - meg比值（0.52）与正常骨髓中的比值（2.3）显著不同，这清楚地表明成人外周血主要携带原始巨核细胞祖细胞。
