Fernando S A, Sportsman J R, Wilson G S
Department of Chemistry, University of Kansas, Lawrence 66045.
J Immunol Methods. 1992 Jul 6;151(1-2):27-46. doi: 10.1016/0022-1759(92)90103-z.
The interactions of two monoclonal antibodies with human growth hormone (hGH) have been investigated. The individual antibodies showed normal behavior in a competitive binding assay, but mixtures of the antibodies demonstrated a 'hook' attributable to cooperative interactions. Cooperativity was observed in titrations which preceded the competitive binding assay. Size exclusion chromatographic data suggest that the cooperativity is explained by the formation of higher molecular weight complexes (up to 700 kDa). The major complex is probably linear, consisting of three antibody molecules. Circular and linear complexes with four antibody molecules (octameric complexes) are also possible. Theoretical models also support the formation of cyclic complexes in a competitive binding assay.
已对两种单克隆抗体与人生长激素（hGH）的相互作用进行了研究。在竞争性结合试验中，单个抗体表现出正常行为，但抗体混合物表现出一种归因于协同相互作用的“钩状效应”。在竞争性结合试验之前的滴定中观察到了协同性。尺寸排阻色谱数据表明，协同性可通过形成更高分子量的复合物（高达700 kDa）来解释。主要复合物可能是线性的，由三个抗体分子组成。具有四个抗体分子的环状和线性复合物（八聚体复合物）也是可能的。理论模型也支持在竞争性结合试验中形成环状复合物。
