National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.
Institute of Consun Co. For Chinese Medicine in Kidney Diseases, C. Consum Pharmaceutical Group, Shenzhen 518000, People's Republic of China.
Int J Nanomedicine. 2019 Sep 20;14:7695-7705. doi: 10.2147/IJN.S218834. eCollection 2019.
Lateral flow assays (LFA) play an increasingly important role in the rapid detection of various pathogens, pollutants, and toxins.
To overcome the drawbacks of low sensitivity and poor quantification in LFA, we developed a new calorimetric LFA (CLFA) using gold nanocages (GNCs) due to their high photothermal conversion efficiency, good stability of photophysical properties, and stronger penetrating ability of NIR light.
Thiol-polyethylene glycol-succinyl imide ester (HS-PEG-NHS) was modified onto GNCs, and the complex was conjugated with an antibody. Subsequently, the antibody-conjugated GNCs were analyzed by UV/Vis spectrophotometer, transmission electron microscope, high-resolution transmission electron microscope with energy dispersive spectrometer, dynamic light scattering instrument, and Atom force microscope. The GNC-based CLFA of alpha-fetoprotein (AFP) and zearalenone (ZEN), a food toxin, required nitrocellulose strips, a NIR laser source, and an infrared camera.
The GNC-labeled CLFA platform technique exhibited detection sensitivity, qualitative specificity, and quantitative accuracy. The superior performance of the technique was evident both in sandwich format detection of biomacromolecules (eg, AFP protein) or competitive format detection of small molecules (eg, ZEN). After optimizing various test parameters, GNC-labeled CLFA provided . 5-6-fold enhanced sensitivity, higher correlativity ( >0.99), and more favorable recovery (82-115%) when compared with visual LFA.
GNC-labeled CLFA may be a promising detection platform with high sensitivity, specificity, and precision.
侧向流动检测（LFA）在快速检测各种病原体、污染物和毒素方面发挥着越来越重要的作用。
为了克服 LFA 灵敏度低和定量能力差的缺点，我们开发了一种新的比色侧向流动分析（CLFA），使用金纳米笼（GNCs），因为它们具有高的光热转换效率、良好的光物理性质稳定性和更强的近红外光穿透能力。
巯基-聚乙二醇-琥珀酰亚胺酯（HS-PEG-NHS）被修饰到 GNCs 上，然后将复合物与抗体结合。随后，通过紫外/可见分光光度计、透射电子显微镜、带能量色散谱的高分辨率透射电子显微镜、动态光散射仪和原子力显微镜对抗体修饰的 GNCs 进行分析。基于 GNC 的 CLFA 用于检测甲胎蛋白（AFP）和食品毒素玉米赤霉烯酮（ZEN），需要硝酸纤维素条、近红外激光源和红外摄像机。
GNC 标记的 CLFA 平台技术表现出检测灵敏度、定性特异性和定量准确性。该技术在生物大分子（如 AFP 蛋白）的夹心式检测或小分子（如 ZEN）的竞争式检测中都表现出了优越的性能。在优化了各种测试参数后，与视觉 LFA 相比，GNC 标记的 CLFA 提供了 5-6 倍的增强灵敏度、更高的相关性（>0.99）和更有利的回收率（82-115%）。
GNC 标记的 CLFA 可能是一种具有高灵敏度、特异性和精度的有前途的检测平台。
